MtoZ Biolabs' Ovary Tissue Protein Extraction Kit is a specialized reagent system for high-yield total protein extraction from structurally complex and hormone-responsive ovarian tissue.
Product Overview
Major challenges of protein extraction from Ovary tissue include:
1. Heterogeneous Follicular Structure
Ovarian cortex, follicles, corpus luteum, and stromal regions contain distinct cell types with different membrane properties and matrix density, leading to uneven lysis and variable protein recovery.
2. Lipid- and Hormone-Rich Microenvironment
Follicular fluid and steroid-producing cells contribute lipids and small hydrophobic molecules that can interfere with solubilization, increase background, and affect downstream electrophoresis or LC-MS analysis.
3. Fragile Cells and Protease Activity
Oocytes and granulosa cells are mechanically delicate, yet once disrupted they can release active proteases that rapidly degrade target proteins during homogenization.
4. Viscosity from Genomic DNA and Matrix Components
Genomic DNA and extracellular matrix fragments released during tissue disruption increase lysate viscosity, complicate pipetting, and can distort electrophoretic band patterns and quantification.
Advantages
1. Balanced Lysis Across Ovarian Compartments
MtoZ Biolabs' Ovary Tissue Protein Extraction Kit uses an optimized lysis buffer that efficiently disrupts follicular, luteal, and stromal regions, supporting thorough cell breakage and robust total protein recovery.
2. Protection of Sensitive Ovarian Proteins
A tailored inhibitor cocktail suppresses ovarian protease activity, helping preserve native protein profiles and post-translational modifications throughout the extraction process.
3. Reduced Lipid and DNA Interference
The extraction workflow is designed to limit lipid carryover and DNA-induced viscosity, generating clarified lysates that are easy to handle and well suited for SDS-PAGE, Western blotting, and LC-MS.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
