MtoZ Biolabs' Desthiobiotin-ADP Probe is a nucleotide analog designed to covalently tag ADP binding proteins in complex lysates, enabling affinity enrichment and unbiased profiling by Western blotting or LC-MS.
Product Overview
Profiling ADP binding proteins in complex samples is difficult, and the main challenges include:
1. State Specific Detection Is Hard
Traditional antibody based or biochemical assays rarely measure ADP bound state directly, making it difficult to distinguish ADP engaged proteins from total protein abundance.
2. No Proteome Wide Co-Capture
In complex proteomes it is difficult to unbiasedly capture multiple ADP binding proteins and their binding competent states at the same time, limiting network level comparisons across conditions.
3. Inhibitor and Ligand Profiling Is Low Throughput
Evaluating selectivity and off target engagement typically requires target by target experiments and can miss unexpected ADP binding proteins.
4. Workflow Disconnects
Labeling and pull down steps are often not fully compatible with downstream WB or LC-MS workflows, which can increase sample loss and background.
Advantages
✔️Active Site Covalent Tagging
Desthiobiotin-ADP is an ADP analog that carries a desthiobiotin handle through a reactive linkage. After endogenous nucleotides are reduced, it selectively forms a covalent adduct at conserved active site lysine residues in the nucleotide binding pocket, directly tagging functional ADP binding sites.
✔️Capture of Active and Inactive Forms
The probe can label and enrich ADP binding competent proteins whether they are catalytically active or temporarily inactive, providing a broader view of ADP pocket states and regulation in complex samples.
✔️Competition Ready Target Engagement Platform
In lysates the probe supports competition formats to compare ligand or inhibitor engagement trends across many ADP binding proteins in parallel for selectivity and off target assessment.
✔️Downstream Ready Compatibility
MtoZ Biolabs' Desthiobiotin-ADP Probe integrates with streptavidin based enrichment and supports SDS-PAGE and Western blot validation as well as LC-MS/MS identification and quantitative profiling.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
