The BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4) from MtoZ Biolabs is a high-purity, deuterated crosslinker designed for efficient covalent protein-protein interaction studies, structural proteomics, and biological conjugation.
Product Overview
BS3-d4 is a powerful crosslinker used in protein-protein interaction studies, but certain challenges still arise when using crosslinkers in experimental workflows:
1. Non-Specific Crosslinking
Crosslinkers with lower purity or reactivity can lead to non-specific interactions, reducing the efficiency of target protein capture and generating complex mixtures that are difficult to analyze.
2. Limited Membrane Penetration
Many crosslinkers have difficulty penetrating cellular membranes, limiting their use for intracellular protein interaction studies or transient protein complex capture in live cells.
3. Inconsistent Weighing
Powdered crosslinkers often require precise weighing, and small errors in measurement can affect experimental reproducibility and accuracy.
4. Limited Application Flexibility
Some crosslinkers may not meet the hydrophobic or hydrophilic requirements needed for various experimental setups, such as crosslinking membrane-bound proteins versus intracellular interactions.
Advantages
The MtoZ Biolabs BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4) offers the following benefits:
✅ High Purity and Reactivity
BS3-d4’s NHS ester groups efficiently react with primary amines to form stable amide bonds, ensuring high specificity and crosslinking efficiency.
✅ Membrane Permeability
As a hydrophobic crosslinker, BS3-d4 penetrates cell membranes, making it suitable for crosslinking proteins in live cells and stabilizing transient or weak protein interactions.
✅ Convenient Pre-Packaged Format
The No-Weigh format eliminates the need for weighing, reducing errors and simplifying the process, ensuring consistent and reliable results every time.
✅ Enhanced Application Flexibility
BS3-d4 is ideal for a wide range of applications, including protein-protein interaction studies, in situ crosslinking, and covalent protein conjugation, applicable to both cell lysates and live cells.
✅ Improved Experimental Precision
The deuterated structure enhances mass spectrometry analysis, allowing for greater accuracy in tracking crosslinker incorporation and protein interactions.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
