MtoZ Biolabs' Recombinant Antibodies are high-purity antibodies produced using recombinant DNA technology in in vitro expression systems such as HEK293 or CHO cells, effectively eliminating the batch-to-batch variability commonly seen in traditional hybridoma production. They help ensure highly consistent experimental results and are widely used in cutting-edge research areas, including proteomics analysis, clinical diagnostics, and biopharmaceutical development.
Product Overview
Recombinant antibodies are antibodies generated through genetic engineering by cloning antibody-encoding gene sequences into expression vectors and transfecting them into host cells such as bacteria, yeast, insect cells, or mammalian cells for in vitro expression and purification. Unlike conventional polyclonal antibodies or hybridoma-derived monoclonal antibodies that rely on animal immune systems, recombinant antibody production is carried out entirely under controlled laboratory conditions.
Recombinant antibodies are defined by confirmed and known antibody gene sequences, including the variable and constant regions of both heavy and light chains. Production typically involves constructing plasmid vectors carrying the target antibody genes and using engineered host cells as production factories. Their core function is to specifically recognize and bind a defined antigen epitope. In research and medicine, the key value of recombinant antibodies lies in their permanence and editability. Once the gene sequence is established, identical antibodies can be produced indefinitely. In addition, genetic engineering enables antibody optimization and customization, such as humanization, affinity maturation, fluorescent labeling, or isotype switching, to meet complex experimental and clinical needs.
Advantages
✅ High Specificity
Recombinant antibodies are produced from selected, optimal gene sequences and can precisely recognize specific antigen epitopes. This high specificity greatly reduces cross-reactivity with off-target proteins, improving data accuracy and making them particularly suitable for complex biological sample analysis.
✅ Outstanding Performance and Sensitivity
Through in vitro selection and engineering optimization, such as phage display, antibody affinity can be enhanced to enable detection of extremely low-abundance target proteins. Recombinant antibodies typically deliver high signal-to-noise and sensitivity across applications including flow cytometry, immunohistochemistry, and Western blotting.
✅ Versatility and Scalability
Recombinant technology provides substantial flexibility. Researchers can readily change antibody formats, such as Fab fragments, scFv, or bispecific antibodies, or switch species origin, such as converting rabbit antibodies to human antibodies. In addition, production is animal-free and can be scaled from milligram to kilogram levels using bioreactors, with shorter timelines and stable yields.
✅ Excellent Reproducibility
Because production is based on defined gene sequences rather than unstable hybridoma cell lines, recombinant antibodies can achieve highly consistent performance across batches, providing reliable support for long-term studies and standardized assays.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
