MtoZ Biolabs' RP-4H HPLC Column is a reverse-phase column built for reliable, high-resolution separations of peptides and protein when you need strong retention, clean peak shapes, and robust reproducibility.
Product Overview
Fast protein LC often faces practical challenges such as:
1. Speed vs Resolution Tradeoff
At higher flow rates, many columns lose resolving power, leading to coelution and unclear protein profiles.
2. High Backpressure at High Flow
Dense packed formats can generate excessive pressure, restricting linear velocity and limiting throughput.
3. Slow Protein Mass Transfer
Large biomolecules diffuse slowly, so porous media can broaden peaks and reduce sensitivity when gradients are shortened.
4. Limited pH and Wash Tolerance
Aggressive cleaning and extreme pH conditions may damage the stationary phase, causing retention drift and shorter column life.
5. Batch and Column Variability
Differences between columns or performance drift over repeated runs can undermine comparability in routine protein workflows.
Advantages
✅ High-Speed Protein Separation
MtoZ Biolabs' RP-4H HPLC Column supports rapid protein separations at elevated linear velocities while maintaining useful resolution for intact protein work.
✅ Low Pressure Operation
High permeability helps keep backpressure manageable, enabling faster methods without pushing instrument limits.
✅ Strong Sensitivity
Efficient mass transfer improves peak shape and signal, supporting protein profiling and LC-MS readouts.
✅ Wide pH Robustness
Broad chemical stability allows more aggressive wash strategies to control carryover and preserve long-term performance.
✅ Reproducible and Rugged Performance
Consistent behavior across runs and strong column-to-column consistency improve reliability for high-throughput protein separations.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
