Targeted Proteomics Service

Targeted proteomics is a scientific discipline focused on the quantitative study of specific target proteins, building on the foundation of general proteomics. Unlike non-targeted proteomics, this approach selectively chooses ions for the collection of mass spectrometry data, enabling the analysis of numerous samples with enhanced accuracy, sensitivity, and reproducibility. Targeted proteomics is applicable to a range of studies including post-translational modifications, protein conformations, protein-protein interactions, dynamics, and the systemic investigation of metabolic and signaling pathways.

 

Targeted proteomics quantitative techniques mainly include multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM). MRM, also referred to as selected reaction monitoring (SRM), employs triple quadrupole mass spectrometry (QQQ-MS) to capture signals conforming to predefined ion criteria, facilitating both relative and absolute quantification of target proteins. PRM extends the capabilities of MRM by integrating the high selectivity of quadrupoles with the superior resolution and precision of the Orbitrap technology. It measures all fragment ions following the fragmentation of selected parent ions, offering improved detection sensitivity and reduced susceptibility to interference.

 



Figure 1. Targeted Proteomics

 

MtoZ Biolabs leverages a high-throughput mass spectrometry platform to offer targeted proteomics analysis services, which include:

1. Parallel Reaction Monitoring (PRM) Services

2. MRM/PRM-Based Quantitative Proteomics Analysis

3. Absolute Quantification Analysis （AQUA）

• • Absolute Quantitative Analysis (AQUA) Service

  AQUA absolute quantitative analysis is a targeted quantitative proteomics technique widely used in various quantitative proteomics studies. The absolute quantification strategy can be used for the absolute quantification of proteins and their modified states.

• • MRM/PRM Quantitative Proteomics Service

  Multiple reaction monitoring (MRM) is a targeted quantitative proteomics research method for target protein. MRM, based on information about the target molecule, selectively chooses data for mass spectrometry data collection, collecting signals that meet the criteria of target ions and eliminating that of ions that do not meet the criteria.