MRM/PRM Quantitative Proteomics Service
MtoZ Biolabs provides MRM/PRM quantitative proteomics service for focused measurement of predefined proteins and peptides, helping researchers verify candidate targets, compare selected proteins across study groups, and extend discovery proteomics findings into targeted quantitative analysis.
MRM and PRM use complementary targeted acquisition strategies to provide selective peptide measurement and high-resolution fragment evaluation, allowing the analytical approach to be matched to target characteristics, sample complexity, and quantitative requirements.
- Complementary MRM and PRM platforms for targeted analysis
- Multiplex measurement of multiple predefined peptide targets
- Stable isotope-labeled standards for absolute quantification
MtoZ Biolabs offers MRM/PRM Quantitative Proteomics Service for focused measurement of predefined proteins and peptides. The service is intended for studies that have moved beyond broad screening and require quantitative verification of selected targets, comparison across experimental groups, or follow-up analysis after discovery proteomics.
Target peptide evaluation, assay development, targeted LC-MS/MS acquisition, quantitative data processing, and quality review can be combined within a single workflow. Stable isotope-labeled peptide standards may also be incorporated when absolute quantification is required and technically appropriate.
Technical Principles
MRM and PRM are targeted mass spectrometry approaches that measure predefined peptide targets using different acquisition strategies.
In MRM, selected precursor and fragment ions are monitored as defined transitions on a triple quadrupole mass spectrometer. This approach is widely used for targeted assays in which the peptides and transitions of interest have already been established. In PRM, selected precursor ions are isolated and analyzed by high-resolution MS/MS. The resulting fragment-ion spectra allow several product ions to be evaluated during data analysis, which is useful for confirming target identity and assessing potential interference.
Method selection depends on the target peptides, sample complexity, number of analytes, available MS evidence, and the required quantitative performance.
MRM/PRM Quantitative Proteomics Service at MtoZ Biolabs
MtoZ Biolabs develops targeted assays around the proteins or peptides that need to be measured.
For MRM analysis, triple quadrupole LC-MS/MS platforms are used for transition-based targeted quantification. PRM workflows are supported by high-resolution mass spectrometry platforms, while high-resolution targeted acquisition can also be incorporated when appropriate. These systems are coupled with Nano-LC separation to accommodate different peptide characteristics and assay requirements.
Our MRM/PRM services may include:
- Target protein and peptide evaluation to identify suitable quantitative peptides
- MRM or PRM assay development and optimization
- Targeted LC-MS/MS analysis of predefined proteins or peptides
- Multiplex analysis of several targets within the same assay when technically feasible
- Stable isotope-labeled peptide standards for selected absolute quantification studies
- Quantitative data processing and quality review
- Project-specific results and data reporting
Candidate proteins from Label-Free, DIA, TMT, iTRAQ, or other proteomics studies can also be transferred into an MRM or PRM assay for focused quantitative confirmation.
Service Advantages
1. Sensitive Targeted Measurement
By concentrating acquisition on selected peptides, MRM and PRM improve measurement of proteins of interest in complex samples and can be particularly useful for relatively low-abundance targets.
2. Multiplex Analysis
Several target peptides can be monitored within the same assay when chromatographic and acquisition conditions permit, allowing efficient evaluation of defined protein panels.
3. Consistent Quantitative Comparison
Peptide selection, acquisition parameters, and quality criteria are considered during assay development to improve consistency across samples and experimental groups.
4. Flexible Quantification Options
MRM and PRM can be used for relative quantitative comparison or combined with stable isotope-labeled standards when an appropriate absolute quantification strategy is needed.
Sample Submission Requirements
For more sample details, please consult our technical team.
FAQ
Q1: How do I choose between MRM and PRM?
MRM is generally preferred for established transition-based assays, while PRM offers high-resolution fragment-ion data for selected precursor ions. The best choice depends on the targets, sample background, assay size, existing MS evidence, and quantitative requirements.
Q2: Can several proteins be measured in the same assay?
Yes. Both MRM and PRM can accommodate multiple targets when suitable peptides and acquisition conditions are available. The practical assay size depends on peptide behavior, chromatographic separation, acquisition timing, and required quantitative performance.
Q3: Can MRM/PRM be used for absolute quantification?
Yes. Stable isotope-labeled peptides can serve as internal standards when an appropriate absolute quantification strategy is established. Peptide selection, calibration design, sample matrix, and sample preparation all need to be considered.
Q4: Is MRM/PRM suitable for low-abundance proteins?
It can be. Targeted acquisition directs measurement toward selected peptides and may improve analysis of relatively low-abundance proteins. Actual detectability still depends on peptide properties, target abundance, sample complexity, and interference.
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Mass Spectrometry Images
4. Raw Data
5. Protein Difference Level Analysis
6. Bioinformatics Analysis
For MRM/PRM analysis, please provide your species, sample type, experimental groups, target protein or peptide list, available proteomics data, and quantitative goal. MtoZ Biolabs can review the targets and recommend a suitable assay strategy.
