Articles

• • Advantages, Disadvantages and Principle of Edman Sequencing

  Edman degradation sequencing, pioneered in the 1950s by Swedish chemist Pehr Edman, is a classic method for determining protein N-terminal sequences. Under mildly alkaline conditions, phenyl isothiocyanate (PITC) selectively reacts with the free α-amino group at the peptide N-terminus, forming a cyclical phenylthiocarbamoyl derivative soluble in organic solvents. Subsequent acid treatment cleaves the terminal amino acid as a detectable anilinothiazolinone (ATZ) derivative, which—after isomerization under ac