Exosomal Sequencing Service

Exosomes are extracellular vesicles that play crucial roles in intercellular communication and signal transduction. Exosome sequencing focuses on sequencing the RNA content within exosomes, including messenger RNA (mRNA), microRNA (miRNA), and long non-coding RNA (lncRNA). These RNAs are key regulators of gene expression, cellular functions, and various biological processes. By employing exosome sequencing, researchers can explore the mechanisms of intercellular communication and the roles of exosomes in both physiological and pathological contexts. Since their initial description in 1983, exosome sequencing technologies have advanced significantly, offering vital insights for diagnostics, prognostics, and the identification of therapeutic targets.

 

MtoZ Biolabs provides a comprehensive exosome sequencing service utilizing the high-throughput Illumina sequencing platform. This service can detect transcription levels at the single nucleotide level across exosomes. In addition to analyzing transcript structures and expression levels, it identifies unknown and rare transcripts, alternative splicing sites, and coding single nucleotide polymorphisms (cSNPs), thus delivering the most extensive transcriptome information available.

 

Analysis Workflow

1. Exosome Isolation

2. RNA Extraction and Purification

3. Sample Quality Control

4. Library Construction

5. High-Throughput Sequencing

6. Data Analysis

 

Service Advantages

1. Experienced technicians provide a full range of professional services, from experimental design and sample testing to data analysis.

2. Clear and efficient processes reduce unnecessary sample and time waste, ensuring short delivery times.

3. MtoZ Biolabs has an independent bioinformatics analysis platform that offers not only routine analysis but also advanced custom analysis.

4. MtoZ Biolabs' multi-omics analysis platform, including proteomics and metabolomics, enables integrated multi-omics analysis, enhancing the quality of publications.

 

Applications

1. Biomarker Discovery

Researchers have successfully identified diagnostic and prognostic biomarkers in areas such as cancer, neurodegenerative diseases, and cardiovascular diseases using exosome sequencing technology.

 

2. Intercellular Communication Research

Exosomes mediate intercellular communication by transmitting signaling molecules. Exosome sequencing technology allows for the study of these signaling molecules, providing deeper insights into the mechanisms of intercellular communication.

 

3. Drug Delivery Systems

Exosomes are frequently used as drug delivery vectors to enhance therapeutic efficacy and reduce side effects. Exosome sequencing technology aids in understanding the behavior and mechanisms of drugs within exosomes.

 

4. Tumor Microenvironment Research

Exosomes can influence the behavior of surrounding cells, promoting tumor progression. Exosome sequencing technology offers better insights into the tumor microenvironment and the role of exosomes within it.

 

Sample Submission Requirements