Exosomal mRNA Sequencing Service

Exosomal mRNA sequencing is a high-throughput analytical technique used to study the mRNA expression profiles and their changes within exosomes. Exosomes are widely present in body fluids and carry various molecules from inside cells, including proteins, RNA, and lipids. By extracting mRNA from exosomes and performing high-throughput sequencing, this service allows comprehensive analysis of the types, abundance, and changes of mRNA within exosomes, revealing the genetic information transferred between cells via exosomes.

 

Exosomal mRNA sequencing service is widely applied in cancer research, immune monitoring, neurodegenerative diseases, and metabolic disease research. In cancer research, this technology helps identify tumor-related exosomal mRNA biomarkers for early diagnosis, monitoring, and treatment response assessment; in immunology, it is used to study the role of exosomes in immune responses; in neuroscience, analyzing the changes in exosomal mRNA helps uncover the pathological processes of neurodegenerative diseases. This service provides strong data support for basic research, disease mechanism exploration, and new drug development.

 

Services at MtoZ Biolabs

Based on high-throughput sequencing technology, MtoZ Biolabs offers an exosomal mRNA sequencing service that extracts mRNA from exosomes and performs high-resolution sequencing to provide researchers with exosomal mRNA expression profiles. Using the advanced Illumina platform for data acquisition ensures highly sensitive and accurate analysis results. This service provides information on the types and abundance of exosomal mRNA, as well as dynamic changes in different biological states, helping to reveal the role of exosomes in intercellular communication, disease mechanisms, and biomarker discovery.

 

Analysis Workflow

1. Sample Collection and Exosome Extraction

Exosomes are extracted from biological fluid samples such as blood, urine, and cell culture supernatants to ensure sample purity and integrity.

 

2. mRNA Extraction and Purification

mRNA is extracted and purified from exosomes, removing interfering substances to ensure the high quality of the extracted mRNA.

 

3. Library Construction and High-Throughput Sequencing

The purified mRNA undergoes library construction and is subjected to deep sequencing using a high-throughput sequencing platform, providing data for the entire transcriptome.

 

4. Data Analysis

Advanced bioinformatics tools are used to process sequencing data, including gene expression analysis, differential analysis, functional annotation, and pathway enrichment analysis.

 

5. Results Interpretation and Reporting

A detailed analysis report is provided, including differentially expressed genes, functional annotation, pathway analysis, and more, helping researchers gain a deeper understanding of the role of exosomal mRNA in biological processes.

 

Service Advantages

1. High Sensitivity and Accuracy

Based on advanced high-throughput sequencing platforms, the service is capable of precisely capturing and quantifying low-abundance mRNA in exosomes, ensuring high accuracy of the data.

 

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

3. Comprehensive Analysis of Exosomal mRNA

By thoroughly analyzing the types, abundance, and expression changes of mRNA in exosomes, this service provides in-depth insights into intercellular communication and biological functions.

 

4. Customized Services

Based on varying research needs, flexible analysis plans are provided to ensure the most relevant and practical research results for clients.

 

Applications

1. Cancer Research

By analyzing the mRNA in exosomes from tumor patients, this service reveals gene expression changes related to tumorigenesis, metastasis, and immune evasion, providing a foundation for early cancer diagnosis and the identification of therapeutic targets.

 

2. Immunomonitoring and Autoimmune Disease Research

The exosomal mRNA sequencing service can help uncover immune system response mechanisms by analyzing changes in exosomal mRNA expression, aiding research into the pathological processes of autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus.

 

3. Neurodegenerative Disease Research

By exploring changes in exosomal mRNA, this service helps uncover the molecular mechanisms behind neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, providing data support for early diagnosis and intervention.

 

4. Drug Development

The exosomal mRNA sequencing service can assess the impact of drugs on intercellular communication and gene expression by analyzing the changes in exosomal mRNA before and after drug treatment, optimizing therapeutic strategies and target identification.

 

Case Study

1. The Landscape of Circular RNAs and mRNAs in Bovine Milk Exosomes

The study aims to investigate the distribution characteristics and potential functions of circular RNA (circRNAs) and mRNA in milk-derived exosomes. The study focuses on exosomes from milk, and high-throughput RNA sequencing technology was used for comprehensive analysis of circRNA and mRNA. The results show that milk exosomes contain a large amount of circRNA and mRNA, and these RNA molecules exhibit high stability. Functional annotation of the exosomal mRNA revealed that these mRNAs are involved in several biological processes, including immune responses, lipid metabolism, and cell signal transduction. Further analysis indicated that these exosomal mRNAs have high stability and can be expressed and perform biological functions in non-bovine systems. The study concludes that mRNA in milk exosomes may play important roles in immune regulation, nutritional support, and disease prevention, and could serve as potential biomarkers or therapeutic targets.

 



Wang, H Y. et al. Journal of Food Composition and Analysis, 2019.

Figure 1. The Venn Diagram Displays the Distribution of 20,118 mRNAs Found in Colostrum and Mature Milk Exosome.

 

Deliverables

1. Comprehensive Experimental Details

 

2. Materials, Instruments, and Methods

 

3. Data Analysis, Preprocessing, and Estimation 

 

4. Bioinformatics Analysis

 

5. Raw Data Files

 

FAQ

Q1: How Does this Service Ensure the Accuracy and Reliability of Sequencing Data?

A1: The service uses a high-throughput sequencing platform combined with strict quality control processes, including quality assessment, library efficiency testing, and sequencing depth optimization. In addition, efficient bioinformatics tools are employed for data cleaning, alignment, and quantification analysis to ensure high accuracy and reproducibility of the data.

 

Q2: Does the Service Support Customized Analysis?

A2: Yes, we offer customized services based on client needs, including targeted analysis of specific genes, enrichment studies of particular biological pathways, and integrated analysis with other omics such as proteomics or metabolomics to meet personalized research requirements.