Full-Length 16S/18S/ITS Sequencing Service

16S/18S/ITS full-length sequencing refers to the process of extracting microbial DNA from samples, using universal primers to amplify the full length of the microbial 16S rDNA, 18S rDNA, or ITS region, and then sequencing it. 16S/18S/ITS full-length sequencing can be used for the detection of microbial diversity.

 

16S rDNA is present in all bacterial genomes and reflects differences between different genera of bacteria. It is widely used for the detection and identification of pathogenic bacteria. 16S rDNA full-length sequencing can be used to analyze the diversity of bacterial community structures. 18S rDNA is the DNA sequence encoding the small subunit rRNA of eukaryotes, structurally divided into conserved regions and hypervariable regions. The conserved regions reflect phylogenetic relationships between species, while the hypervariable regions reflect differences between species. Full-length sequencing of 18S rDNA can be used to analyze the community structure of eukaryotic microorganisms. ITS is the intergenic spacer sequence between 18S rDNA and 28S rDNA in eukaryotes, and ITS full-length sequencing can be used for fungal community structure analysis.

 

Service Advantages

1. Experienced Technical Team

Offers comprehensive professional services from experimental design through sample testing to data analysis. 

 

2. Streamlined Procedures

Minimizes unnecessary sample usage and time waste, ensuring faster turnaround times.

 

3. Customized Strategies

Employs varied extraction methods and sequencing strategies tailored to different sample sources to accommodate diverse environmental studies.

 

4. Integrated Multi-Omics Analysis

MtoZ Biolabs utilizes advanced platforms for proteomics and metabolomics, enhancing paper quality through comprehensive omic integration.

 

Analysis Workflow

1. 16S/18S/ITS Full-Length Sequencing Process

 



Figure 1. 16S/18S/ITS Full-Length Sequencing Process

 

2. Data Analysis Process

 



Figure 2. Data Analysis Process

 

Sample Submission Requirements

Types of samples: Meta samples such as feces, soil, etc. or Meta DNA samples can be tested. For detailed requirements, please consult our technical staff.