Protein Identification
Overview
MtoZ Biolabs utilizes 2D-nano LC-MS/MS technology for protein identification. In our general workflow, protein complexes are separated in 1D/2D gel and the target protein is then digested into peptide fragments, followed by HPLC separation and tandem MS analysis. We use Scaffold and Mascot software for analyzing peptide MS data, and ensuring confident protein identification.
Service Workflow
Sample Requirements
| Format | Gel bands, gel spots, and liquid samples are acceptable. |
| Quantity | Gel bands/spots that are visible to naked eyes are adequate for protein identification. For liquid samples, a total of 10 ug proteins are required. |
| Purity | Purity of liquid samples should be as high as possible. For liquid sample <10 ug, please avoid large amounts of detergent and salt ions, and note the buffer composition and the estimated amount of total proteins when submitting samples. |
| Note | All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated. |
Reports
• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensity
• Protein identifications and intensity
Related Services
Protein Identification
Protein Mass Measurement
LC-MS Analysis of Pull-down Proteins
Native MS Analysis
Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-length Sequencing
Phospho-proteomics
Acetyl-proteomics
Ubiquitin-proteomics
Glyco-proteomics
Disulfide bond
Histone Modifications
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Accurate mass determination can be applied to all areas encompassed by chemistry and Accurate mass determination can be applied to all areas invol
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• Protein Structure Identification
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• Protein Mass Spectrometry Identification
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