Protein Mass Measurement

Overview

Molecular mass is the most fundamental characteristics for proteins and peptides. It directly reflects the structural integrity of proteins, and hence it is commonly applied in identification of proteins and peptides. To better assist the scientific research of our clients, MtoZ Biolabs provides macromolecular mass measurement service based on Bruker ultrafleXtreme™ MALDI TOF/TOF, and high-resolution protein mass measurement service via nano LC-MS. This service can be applied to accurately analyze the molecular mass, subunits, and dimerization/polymerization of multiple biotechnological molecules, including proteins, peptides, antibodies, and vaccines, etc.

Analytical Technique

image-Protein-Mass-Measurement-Bruker-ultrafleXtreme-MALDI-TOF-TOF.png

Application

• Measurement of the molecular mass of protein/peptide/antibody/vaccine, etc.
• Analysis of number of subunits in protein sample
• Analysis of dimerization/polymerization of protein sample
• Evaluation of sample purity and protein/peptide contaminants

Sample Requirements

Format	Liquid and Gel samples are acceptable.
Quantity	A total of 10-50 ug proteins are required.
Purity	Purity of protein sample should be >90%; Detergents, such as SDS and Triton-X, salt, Tris, and other contaminants should be as low as possible.
Note	All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated.

*Customers are recommended to contact us for detailed sample requirements before sending your samples.

Case Study

In this study, the molecular mass of the protein sample from one of our customer was analyzed by MALDI-TOF. The result below showed a very significant peak at 1046.6 Da with intensity of nearly 100%, which indicated the protein mass was 1046.6 Da with high purity. The measured protein mass was consistent with the theoretical value.

Reports

• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensity
• Protein identifications and intensity