Resources

Proteomics Databases



Metabolomics Databases

• • Common Pitfalls and Optimization Strategies in Shotgun Proteomics Analysis

  Shotgun proteomics is one of the core technologies in modern proteomics research. Owing to its high-throughput capacity and broad proteome coverage, it plays an important role in many areas of the life sciences. However, in practical applications, researchers often encounter challenges related to sample preparation, instrument configuration, and data analysis, which may affect the depth of proteome coverage, data accuracy, and reproducibility. This article systematically summarizes common misconceptio......

• • What Is Site-Specific Histone Ubiquitination Analysis?

  Histone ubiquitination is an important epigenetic modification that regulates chromatin structure and gene expression through the covalent attachment of the small regulatory protein ubiquitin to lysine residues on histones. Ubiquitination at different histone sites has distinct functions in transcriptional regulation, chromatin remodeling, and DNA damage repair, and its role in gene silencing or activation often depends on the modification status of specific sites. Site-specific histone ubiquitination......

• • Western Blot Housekeeping Protein: Principles, Applications, and Method Considerations

  Technical guide for Western Blot Housekeeping Protein: Principles, Applications, and Method Considerations.

• • Antibody Characterization Methods: Principles, Applications, and Method Considerations

  Technical guide for Antibody Characterization Methods: Principles, Applications, and Method Considerations.

• • Biomarkers In Medicine: Principles, Applications, and Method Considerations

  Technical guide for Biomarkers In Medicine: Principles, Applications, and Method Considerations.

• • Confirming Mature Protein Starts: N-Terminal Sequencing for Processing Site Verification and Lot Release

  Teams evaluating whether a sample can support reliable N-terminal confirmation should define the QC question, expected read length, and documentation standard before choosing a specific analytical route. MtoZ Biolabs can Review N-terminal sequencing feasibility across Edman and MS workflows before samples are prepared or submitted.

• • How Much Does Edman Sequencing Cost? Factors That Influence Cycle Number and Sample Preparation

  A three-cycle confirmation on clean synthetic peptide is a different workload from a fifteen-cycle read on legacy recombinant protein with suspected N-terminal modification and documentation- grade reporting. Treating these projects as equivalent leads to under-budgeting, repeat runs, or a report that does not support the intended QC decision.

• • Edman Sequencing vs LC-MS/MS: Which Method Better Answers Your N-Terminal Sequence Question?

  Edman sequencing and LC-MS/MS can both contribute to N-terminal characterization, but they answer slightly different questions. Edman degradation provides a direct, cycle-based read from the free N-terminus of purified protein or peptide material. LC-MS/MS analyzes N-terminal peptides, often after digestion or enrichment, and interprets mass spectrometric evidence in a broader analytical context.

• • How to Prepare a Protein Sample for Edman Sequencing: Purity, Transfer, and N-Terminal Accessibility

  Edman sequencing failures often begin before the sequencer starts. A project may have a valid N- terminal question and an acceptable sample amount, yet still return weak cycle-one signal, ambiguous early residues, or a shorter read than planned. In many cases, the problem is not the instrument. It is sample preparation. Purity, transfer quality, loading format, and N-terminal accessibility all determine whether the chemistry can read the first residues reliably.

• • How to Detect Low-Abundance Histone Malonylation?

  Lysine malonylation (Kmal) is an important type of post-translational modification that has attracted increasing attention in studies of metabolic regulation, epigenetics, and disease mechanisms in recent years. Because Kmal is closely associated with intracellular malonyl-CoA levels, it can translate metabolic status into regulatory information related to chromatin structure and gene expression, and is therefore considered an important bridge between metabolism and epigenetics. However, in practical ......