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Proteomics Databases
Metabolomics Databases
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Glycosylation is one of the most widespread post-translational modifications in eukaryotic proteins and plays integral roles in cellular communication, receptor recognition, immune regulation, tumorigenesis, and other biological processes. As two major forms of glycosylation, N-glycosylation and O-glycosylation share the same broad modification category but differ substantially in modification mechanisms, structural diversity, and analytical strategies. This article systematically compares the quantit......
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Glycosylation is one of the most widespread protein post-translational modifications and plays critical roles in multiple biological processes, including cellular recognition, signal transduction, and immune regulation. Glycoproteomic research not only facilitates a deeper understanding of the intricate regulatory mechanisms of biological systems but also shows substantial potential in elucidating disease mechanisms and discovering biomarkers. Advances in mass spectrometry have provided essential tech......
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• PhIP-Seq: What It Is and When Researchers Should Use It
Phage immunoprecipitation sequencing, usually referred to as PhIP-Seq, was developed for this type of question. Instead of testing one antigen at a time, PhIP-Seq uses large phage-displayed peptide libraries to profile antibody binding at scale.
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• Applications of iTRAQ-Based Peptidomics in Inflammation Research
iTRAQ is an isobaric isotope-labeling strategy that covalently labels peptide N-termini and lysine side chains. Through 4-plex or 8-plex multiplex labeling, iTRAQ enables simultaneous relative quantification of multiple samples. In tandem mass spectrometry (MS/MS), these isobaric tags fragment to generate characteristic reporter ions, allowing accurate comparison of peptide abundance across different treatment groups. iTRAQ-based peptidomics has been widely applied in inflammation research, including ......
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• Understanding the Potential of Peptidomics in Cancer Detection
Peptidomics, a mass spectrometry-driven branch of life science research, enables comprehensive analysis of naturally occurring short peptides and endogenous peptide fragments in vivo. These peptides often carry disease-specific molecular information and are emerging as promising biomarker sources for early cancer diagnosis and therapeutic response monitoring. This article systematically examines the potential of peptidomics in cancer detection from the perspectives of technical foundations, frontier a......
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• What is Histone Malonylation and How Does It Work?
In life science research, histone modifications have long been central to epigenetic studies. Histones, as the primary structural proteins of chromatin, undergo chemical modifications that not only regulate chromatin compaction but also influence gene expression, cell differentiation, and disease progression. In recent years, a novel modification termed histone propionylation (Hpr) has emerged as a prominent focus of research. Basic Concept of Histone Propionylation Histone propionylation is an acyla......
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• How to Identify Disease Biomarkers Using Exosome Proteomics?
In precision medicine and early disease diagnosis research, exosome proteomics has emerged as a crucial tool for discovering reliable biomarkers. Exosomes are extracellular vesicles with diameters of 30-150 nm that carry proteins, nucleic acids, lipids, and other molecules, reflecting the physiological and pathological state of their originating cells. Unique Advantages of Exosomes in Disease Research Exosomes possess several key characteristics that make them ideal candidates for biomarker studies: ......
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• What is Histone Propionylation and What Are Its Biological Functions?
Within the complex epigenetic regulatory network, post-translational histone modifications continue to be identified, greatly expanding our understanding of gene expression regulation. Following classical modifications such as acetylation and methylation, histone propionylation, a metabolite-derived modification, has recently attracted increasing attention. This modification not only links cellular metabolic states to chromatin structural dynamics but also plays distinct roles in transcriptional regul......
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• What Is the Complete Workflow of Histone Propionylation Analysis?
With the increasing depth of research in epigenetic regulation, histone post-translational modifications (PTMs) have emerged as a critical approach for elucidating mechanisms of gene expression regulation. In recent years, beyond classical modifications such as acetylation and methylation, histone propionylation (Kpr) has gained considerable attention as an emerging modification due to its close association with cellular metabolic states. As a key metabolic intermediate, fluctuations in propionyl-CoA ......
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• How Is Histone Butyrylation Linked to Short-Chain Fatty Acid Metabolism?
With the rapid advancement of epigenetics, histone post-translational modifications (PTMs) have emerged as a central avenue for elucidating mechanisms of gene expression regulation. In recent years, histone butyrylation (Kbu), an emerging type of acylation modification, has attracted increasing attention in the life sciences. Concurrently, short-chain fatty acids (SCFAs), key metabolites produced by the gut microbiota, are increasingly recognized for their roles in host metabolism and epigenetic regul......
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