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Proteomics Databases



Metabolomics Databases

• • What Is the Relationship Between Histone Acetylation and Gene Activation?

  Histone acetylation is one of the most classical and representative post-translational modifications in epigenetic regulation and exhibits a strong positive correlation with gene expression activation. In eukaryotic cells, DNA does not exist in a naked form but is wrapped around histone octamers to form nucleosome structures. Histone acetylation promotes transcription initiation and elongation at multiple levels by altering chromatin architecture and modulating the recruitment efficiency of the transc......

• • Targeted Metabolomics Analysis Process

  Targeted metabolomics has emerged as a core approach for investigating dynamic changes in biological systems, particularly in precision medicine, drug development, and mechanistic studies. Owing to its high sensitivity, quantitative accuracy, and reproducibility, targeted metabolomics is widely used for quantitative analyses and mechanistic validation. This article provides a systematic overview of the complete workflow of targeted metabolomics, aiming to help researchers understand critical technical......

• • Untargeted Metabolomics: Sample Collection, Processing, and QC

  With the rapid advancement of systems biology research, untargeted metabolomics has become a crucial tool for investigating dynamic changes in biological systems. Unlike targeted approaches, untargeted metabolomics aims to comprehensively capture metabolite profiles within samples, enabling biomarker discovery, metabolic pathway elucidation, and studies of disease mechanisms. However, the extensive diversity and varying physicochemical properties of metabolites mean that every step in the experimental......

• • TMT-Based Quantitative Proteomics in Cancer Research

  Cancer is a highly heterogeneous and complex disease, involving biological processes at multiple levels, including gene mutations, regulation of protein expression, and dysregulation of signaling pathways. Although high-throughput transcriptomic techniques such as RNA-Seq have been extensively applied in cancer research, transcriptional profiles do not fully reflect protein expression or functional states. TMT-based quantitative proteomics, a relative quantification approach utilizing isobaric mass ta......

• • Comprehensive Host Cell Protein (HCP) Analysis for Safer Biologic Development

  With the rapid expansion of biopharmaceutical modalities, including antibody therapeutics, recombinant proteins, vaccines, and cell and gene therapies, host cell proteins (HCPs), as process-related impurities, represent a critical quality attribute influencing product quality, safety, and regulatory compliance. HCPs may induce immunogenicity, degrade active pharmaceutical ingredients (APIs), interfere with pharmacological activity, or compromise product stability. Consequently, systematic strategies f......

• • Host Cell Protein Antibody Coverage Analysis

  During biopharmaceutical development, host cell proteins (HCPs), as process-related impurities, may trigger immune responses, compromise therapeutic efficacy, and potentially induce adverse effects if present in the final drug product. Consequently, regulatory agencies worldwide, including the EMA and NMPA, require rigorous monitoring and control of HCP-related analytical performance throughout biopharmaceutical manufacturing. Currently, enzyme-linked immunosorbent assay (ELISA) is widely accepted as ......

• • The Crucial Role of Mass Spectrometry in Acetylproteomics: From Identification to Quantification

  Protein post-translational modifications (PTMs) constitute a central mechanism for regulating cellular functions. Among them, acetylation, one of the most prevalent PTMs, plays critical roles in gene expression regulation, chromatin remodeling, and metabolic processes. As proteomics research advances, systematic identification and quantification of acetylation are essential to elucidate its mechanistic roles in disease progression, cellular stress responses, and drug effects. Mass spectrometry (MS), a......

• • LC‑MS/MS Techniques for Glycosylation Site Occupancy Quantification

  Glycosylation is one of the most prevalent and structurally complex post-translational modifications in eukaryotic organisms. A substantial body of evidence has demonstrated that glycans are not only involved in protein folding, secretion, and intercellular recognition, but also play critical roles in regulating protein biological activity, stability, and immune-related functions. In studies of antibody therapeutics, recombinant protein drugs, and disease biomarkers, in addition to glycan structural c......

• • How to Analyze Histone Succinylation Sites?

  Histones are core structural components of chromatin, and diverse covalent modifications of lysine residues within histones play critical regulatory roles in gene expression and chromatin dynamics. Succinylation (Ksucc), an emerging histone modification, involves the covalent addition of negatively charged succinyl groups to lysine residues. This modification not only markedly alters the electrostatic properties of nucleosomes and chromatin accessibility, but also links cellular metabolic status to ep......

• • How to Quantify Histone Succinylation Levels?

  Histone succinylation is an important post-translational modification that regulates chromatin structure and gene expression through the covalent addition of succinyl groups to lysine residues. This modification not only alters the electrostatic properties of nucleosomes and promotes chromatin accessibility, but also participates in transcriptional activation, DNA damage repair, and cellular metabolic regulation. Quantitative analysis of histone succinylation levels enables precise assessment of gene ......