Resources
Proteomics Databases
Metabolomics Databases
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Shotgun protein sequencing is a prominent technique utilized in proteomics for the rapid and comprehensive analysis of complex protein mixtures. Analogous to shotgun genomic sequencing, this method focuses on the identification and quantification of all proteins within a sample through random digestion and subsequent analysis. The technique's core involves the use of mass spectrometry to analyze peptide fragments produced by enzymatic digestion of proteins. Its applications are extensive, spanning bas......
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Phosphorylation detection is a technique for studying the phosphorylation state of proteins. As one of the most common and significant post-translational modifications (PTMs), phosphorylation regulates protein functions by adding phosphate groups to specific amino acid residues such as serine, threonine, and tyrosine. This modification is facilitated by protein kinases, while phosphatases catalyze dephosphorylation. Due to its dynamic and reversible nature, phosphorylation plays a crucial role in vari......
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• Quantitative Mass Spectrometry in Proteomics
Quantitative mass spectrometry in proteomics is a cornerstone technology in modern life sciences, designed to quantitatively detect and identify proteins within biological samples using mass spectrometry techniques. The advancement of this technology offers novel approaches to understanding the complex dynamics of biological systems. In biomedical research, examining protein expression levels, post-translational modifications, and their changes is vital for elucidating the molecular mechanisms underpi......
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Yeast 2 hybrid analysis is a molecular biology technique used to study protein-protein interactions. First developed by Fields and Song in 1989, the method exploits the transcription activation mechanism in yeast cells to detect whether two proteins are capable of interacting with each other. The basic principle of yeast 2 hybrid analysis involves fusing the proteins of interest to the activation domain and DNA binding domain of a transcription factor. When expressed in yeast cells, if the two protein......
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• Multiple Sequence Alignment Analysis
Multiple sequence alignment analysis is a bioinformatics technique used to compare multiple biological molecular sequences (such as DNA, RNA, or protein sequences) in order to identify their similarities and differences. By comparing sequences from different species or individuals of the same species, this technique can reveal evolutionary relationships, identify functionally conserved regions, and discover potential functional variations. For example, in proteomics research, it helps scientists ident......
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GC-MS amino acid analysis is a method employing gas chromatography-mass spectrometry (GC-MS) technology for the qualitative and quantitative analysis of amino acids. This technique is particularly suitable for analyzing amino acids in complex biological samples. Amino acids are the fundamental building blocks of proteins and also serve as key molecules in many metabolic pathways within organisms. Accurate analysis of the types and concentrations of amino acids is of significant importance in life scie......
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• FTIR Analysis of Protein Structure
FTIR analysis of protein structure is an analytical technique used to study the secondary structure and conformational changes of proteins. By performing FTIR analysis, vibrational information about various chemical bonds in protein molecules can be obtained, allowing the structural features of the protein to be inferred. This method is particularly suitable for investigating conformational changes in proteins under different environmental conditions, such as variations in temperature, pH, or solvent ......
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• Drug Target Identification Methods
Drug target identification methods play an essential role in modern medical research. These techniques not only help uncover the molecular mechanisms of diseases but also provide the core foundation for the development of new drugs. Drug target identification methods primarily include various techniques, such as computational biology, proteomics, chemical biology, and systems biology. Computational biology predicts potential drug targets by analyzing large-scale data and applying algorithmic models. I......
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Signal peptide analysis is mainly applied to investigate the role of signal peptides in protein synthesis and secretion. A signal peptide is a short amino acid sequence, typically located at the N-terminus of newly synthesized proteins, that directs the cellular localization and secretion pathways of these proteins. The primary function of the signal peptide is to guide proteins across the cell membrane into organelles such as the endoplasmic reticulum, mitochondria, or chloroplasts, or to facilitate ......
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• Drug Target Identification and Validation
Drug target identification and validation are integral steps in the drug development process. A drug target refers to a biological molecule or a group of molecules that play a crucial role in the onset and progression of a disease. Drugs achieve therapeutic effects by interacting with these targets. Validation confirms the function and biological significance of the identified targets, ensuring they can produce the anticipated therapeutic effects in both in vitro and in vivo experiments. The importanc......
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