Resources

Proteomics Databases



Metabolomics Databases

• • Histone Methylation Quantitative Proteomics by LC-MS/MS

  Quantitative LC-MS/MS analysis of histone methylation sites including mono-, di-, and tri-methyl states with antibody enrichment.

• • Quantitative Enzyme Activity Analysis by ABPP and LC-MS/MS

  Quantify active enzymes with activity-based probes, enrichment, and LC-MS/MS using TMT or label-free workflows.

• • Label-Free Quantitative Proteomics for Protein Interactions

  Label-free LC-MS/MS quantification applied to Co-IP and interaction proteomics without isotope tags.

• • Absolute vs Relative Quantitative Proteomics Explained

  Compare absolute and relative quantitative proteomics: isotope standards, SWATH, label-free, and TMT/iTRAQ methods.

• • How to Use GFP Tags for Co-Immunoprecipitation

  GFP-tag Co-IP using GFP-Trap beads, gentle lysis, wash optimization, and optional LC-MS/MS for interactome discovery.

• • Chemoproteomics: Principles, Workflow, and Applications

  Chemoproteomics uses activity-based probes and LC-MS to map protein–small-molecule interactions, targets, and enzyme activity.

• • Histone Phosphorylation in Epigenetic Regulation

  Definition and roles of histone phosphorylation in transcription, DNA damage response, and mitosis, plus MS detection approaches.

• • How to Analyze Histone Phosphorylation Using Mass Spectrometry

  LC-MS/MS workflow for histone phosphorylation: acid extraction, propionylation, phosphopeptide enrichment, and site localization.

• • Acetylation Site Analysis by Acetyl-Proteomics LC-MS/MS

  Workflow for acetylation site analysis: enrichment, LC-MS/MS, and site localization by acetyl-proteomics.

• • Exosome Proteomics: Overview and Workflow

  Overview of exosome proteomics: EV isolation, LC-MS/MS quantification, and biomarker applications.