Resources

Proteomics Databases



Metabolomics Databases

• • What Is Histone Crotonylation Analysis?

  Histone post-translational modifications (PTMs) represent a key mechanism regulating chromatin structure and gene expression. In recent years, with advances in mass spectrometry technologies, histone crotonylation (Kcr), a novel acylation modification, has attracted increasing attention. As a short-chain unsaturated acyl modification occurring on lysine residues, crotonylation is structurally distinct from classical acetylation and exhibits unique biological functions in transcriptional regulation, ce......

• • Advantages of SILAC Labeling Coupled with Mass Spectrometry in Protein Expression Research

  Accurate quantification of protein expression is essential for elucidating cell signaling, disease mechanisms, and drug targets. However, protein expression is regulated through complex, multilayered, and dynamic processes, and conventional methods such as Western blotting alone are often inadequate to meet the demands of high-throughput analysis, quantitative accuracy, and cross-sample comparability. As a metabolic labeling strategy, SILAC (Stable Isotope Labeling by Amino acids in Cell culture), whe......

• • Exosome Proteomics: Key Techniques and Applications in Research

  Exosomes are small extracellular vesicles with diameters of approximately 30-150 nm, widely present in biological fluids such as blood, urine, and saliva. As key mediators of intercellular communication, exosomes carry biomolecules including proteins, nucleic acids, and lipids, and play critical roles in disease diagnosis, drug target identification, and the optimization of therapeutic strategies. In recent years, with advances in mass spectrometry and proteomics, exosome proteomics has emerged as a m......

• • Antibody Mass Spectrometry Characterization: Principles, Workflow, and Applications

  In the context of the rapid advancement of biopharmaceutical research and development, antibody-based therapeutics (particularly monoclonal and bispecific antibodies) have become a central component of innovative drug development. Concurrently, increasing demands have been placed on the precise structural characterization and quality control of antibodies. As a high-resolution and high-sensitivity analytical technique, antibody mass spectrometry characterization is progressively emerging as a core too......

• • Applications and Value of PRM Mass Spectrometry in Protein Quantification

  Although immunoassay-based methods such as ELISA and Western blot are widely employed, they exhibit inherent limitations in specificity, throughput, and quantitative accuracy. These constraints become particularly evident when analyzing complex biological matrices and low-abundance proteins. Consequently, there is a growing demand for analytical approaches that offer high sensitivity, robust reproducibility, and accurate quantification to support applications such as biomarker validation, drug target ......

• • Advantages and Disadvantages of High-Throughput Ubiquitinomics

  Ubiquitination, as one of the most important post-translational modifications, is widely involved in biological processes such as cell cycle regulation, DNA repair, signal transduction, and protein degradation, and serves as a central mechanism for maintaining cellular homeostasis and regulating signaling pathways. However, ubiquitination is characterized by low abundance, rapid turnover, and complex chain architectures, which have long hindered its systematic and high-resolution characterization. Tra......

• • Beginner’s Guide to Acetylproteomics Research: Sample Prep and Analysis Tips

  As a major type of protein post-translational modification (PTM), acetylation is extensively involved in fundamental biological processes, including cellular metabolism, signal transduction, and gene expression regulation. In particular, in studies of histone regulation, mitochondrial function, and metabolic reprogramming, protein acetylation has emerged as a cutting-edge area for elucidating disease mechanisms and identifying potential therapeutic targets. With advances in high-resolution mass spectr......

• • Mechanisms and Pathways of Protein Ubiquitination

  Within complex cellular systems, the dynamic regulation of protein function represents a fundamental mechanism for maintaining cellular homeostasis. Protein ubiquitination, a highly conserved and reversible post-translational modification, is broadly involved in diverse biological processes, including cell cycle regulation, stress responses, DNA repair, and signal transduction. By covalently tagging substrate proteins, ubiquitination governs their stability, activity, subcellular localization, and int......

• • How to Enrich Golgi Proteins for Proteomic Analysis?

  The Golgi apparatus, a central membranous organelle within cells, plays essential roles in protein modification, sorting, and transport. It is critical for cellular signaling, the secretory system, and understanding disease mechanisms. Therefore, precise identification and quantitative analysis of Golgi proteins are crucial for elucidating cellular functions and disease processes. However, due to the low abundance of Golgi proteins within total cellular proteins, their enrichment and isolation remain ......

• • How to Enrich Lactylated Peptides for Mass Spectrometry?

  Lactylation is an emerging post-translational modification, first identified in 2019, which modulates protein structure and function by introducing a lactyl group (-CO-CH₃-OH) onto lysine residues. Lactylation plays critical roles in regulating carbohydrate metabolism, inflammatory responses, and tumorigenesis. For instance, histone lactylation can modulate chromatin accessibility, thereby influencing gene expression, while non-histone lactylation participates in the regulation of cellular metabolic p......