Resources
Proteomics Databases
Metabolomics Databases
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• Can PhIP-Seq Detect Conformational or Modified Autoantigens?
A technical guide explaining whether PhIP-Seq can detect conformational or modified autoantigens, covering library design limits, PTM variant screening, and validation paths for autoimmune serology.
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Plan a polyclonal antibody sequencing project by addressing sample purity, reference data integration, and validation tiers before MS analysis begins.
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A structured checklist of sample, data, and reporting questions to confirm before selecting a protein mass spectrometry service provider.
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• High Throughput MS Profiling
Learn how high throughput MS profiling supports proteomics, metabolomics, and lipidomics cohorts with standardized LC-MS workflows and reproducible outputs.
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• Shotgun vs Targeted Mitochondrial Proteomics: How Do They Compare?
Mitochondria are the cellular powerhouses responsible for ATP production, regulation of apoptosis, and participation in metabolic signaling pathways. With advancements in mass spectrometry, mitochondrial proteomics has become a crucial tool for investigating cellular energy metabolism, neurodegenerative diseases, tumor metabolism, and drug mechanisms. In proteomics studies, researchers typically choose between two main strategies: the Shotgun (Global Proteome Scanning) Approach and the Targeted Approa......
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• How to Analyze Mitochondrial Membrane Proteins via LC-MS/MS?
Mitochondria, as central organelles in cellular energy metabolism and signal regulation, rely heavily on the precise regulation of membrane proteins for their functions. Mitochondrial membrane proteins are involved not only in oxidative phosphorylation and metabolite transport but also play critical roles in processes such as apoptosis and immune regulation. However, due to their high hydrophobicity, low abundance, and complex membrane architecture, proteomic analysis of mitochondrial membrane protein......
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• How Does Histone Kbhb Interact With Acetylation and Crotonylation?
In the context of dynamically changing cellular metabolic states, histone post-translational modifications are increasingly recognized as key hubs linking metabolism and the regulation of gene expression. Among them, lysine β-hydroxybutyrylation (Kbhb), a modification derived from ketone metabolism, can directly respond to changes in nutrient availability and modulate gene transcription at the chromatin level. Concurrently, classical acetylation (Kac) and the more recently highlighted crotonylation (K......
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• How to Deparaffinize FFPE Tissue Samples for LC-MS Proteomics?
In clinical research and translational medicine, formalin-fixed paraffin-embedded (FFPE) tissue samples are highly valuable biological resources due to their long-term stability and comprehensive clinical information. However, before FFPE samples can be analyzed by LC-MS proteomics, a key technical challenge must be addressed: efficient and reproducible deparaffinization. Why Must FFPE Samples Be Deparaffinized? During FFPE sample preparation, tissues undergo formalin fixation and paraffin embedding.......
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• Workflow for TMT-labeled Quantitative Mass Spectrometry
As life science research moves toward higher-resolution and large-scale, systems-level investigations, quantitative proteomics has become an essential approach for characterizing dynamic biological changes. In particular, mass spectrometry (MS)-based quantification has demonstrated substantial utility in studies of tumor heterogeneity, investigations of drug mechanisms of action, and discovery of disease biomarkers. Among available strategies, tandem mass tag (TMT) labeling is widely adopted for multi......
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• How to Improve LC-MS/MS Proteomics Data Quality: From Sample Preparation to Peptide Identification
Learn how to improve LC-MS/MS proteomics data quality from sample preparation through peptide identification, including digestion control, acquisition depth, database setup, and PSM review.
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