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Proteomics Databases



Metabolomics Databases

• • What Are the Technical Challenges in Histone Propionylation Analysis?

  Histone propionylation, an important type of short-chain lysine acylation on histones, has increasingly attracted research interest in studies of epigenetic regulation and its coupling with cellular metabolism. This modification has been implicated in the regulation of chromatin structure, control of gene transcription activity, and cellular responses to metabolic states. However, because histone propionylation is typically of low abundance and structurally very similar to acetylation, butyrylation, a......

• • What Is H3K27ac and Why Is It a Popular Epigenetic Mark?

  In complex gene regulatory networks, the DNA sequence alone cannot fully account for the dynamic changes in gene expression, and epigenetic modifications play a crucial role. Histone modifications, as a major form of epigenetic regulation, directly influence gene expression by altering chromatin structure and modulating transcription factor accessibility. Notably, H3K27ac (acetylation of lysine 27 on histone H3) has become a key marker in functional genomics research due to its strong association with......

• • How to Isolate and Enrich Mitochondria for Proteomics Analysis?

  In modern life science research, mitochondria are recognized not only as cellular powerhouses but also as central regulators of cellular metabolism, apoptosis signaling, and disease development. Advances in mass spectrometry have enabled detailed analyses of the mitochondrial proteome, metabolome, and lipidome. However, the low abundance of mitochondria within cells and the presence of complex cellular backgrounds make mitochondrial enrichment a critical step for high-quality mass spectrometry analysi......

• • What Is Histone Acetylation Analysis?

  Histone acetylation refers to the post-translational addition of acetyl groups to lysine residues on histone proteins catalyzed by histone acetyltransferases. This modification alters chromatin structure and regulates gene transcriptional activity. By neutralizing the positive charge of lysine residues, histone acetylation weakens the electrostatic interaction between DNA and histones, leading to a transition of chromatin from a condensed to an open configuration. As a fundamental component of epigene......

• • How to Analyze Histone Acetylation Sites by Mass Spectrometry?

  In epigenetic research, histone acetylation is a key post-translational modification (PTM) that regulates chromatin accessibility and gene expression. The composition and dynamic changes of acetylation sites directly reflect the transcriptional regulatory state of cells. Therefore, achieving high-sensitivity and high-coverage analysis of acetylation sites has become a critical technical challenge at the intersection of proteomics and epigenetics. Mass spectrometry has emerged as one of the most powerf......

• • Lactylation Proteomics Analysis: A Comprehensive Workflow from Enrichment to Identification

  Lactylation (Lysine Lactylation, Kla) is an important post-translational modification discovered in recent years and has been demonstrated to play critical roles in gene expression regulation, immunometabolism, tumor biology, and related fields. However, as a low-abundance and technically challenging acylation modification, lactylation research imposes higher demands on experimental methodologies. How can lactylation sites be accurately captured from complex proteomes? How can high-throughput, high-re......

• • How to Perform Histone Propionylation Proteomics Analysis?

  Histone propionylation is an important post-translational modification that plays a critical role in regulating gene expression, chromatin remodeling, and the pathogenesis of various diseases. With advances in proteomic technologies, researchers can systematically characterize the distribution and functional implications of histone propionylation using high-throughput proteomic approaches, providing valuable data for precision medicine research. Scientific Background of Histone Propionylation Proteom......

• • How to Detect Low-Abundance Histone Propionylation?

  Histone propionylation is a critical epigenetic modification that plays an important role in gene regulation. However, its cellular abundance is typically low, posing significant challenges for detection and quantification. To address this issue, researchers have developed a range of reliable detection methods by integrating highly sensitive mass spectrometry techniques, peptide enrichment strategies, and optimized experimental designs. Challenges in Detecting Low-Abundance Histone Propionylation His......

• • How to Apply Exosome Proteomics in Cancer Research?

  With the advancement of precision medicine, cancer research is increasingly transitioning from single-cell analysis to multi-omics studies of biofluids. Exosomes, as natural carriers of intercellular information, hold significant potential in early cancer diagnosis, identification of therapeutic targets, and investigations of the tumor microenvironment through their proteomic content. Definition of Exosomes and Their Significance in Cancer Research Exosomes are small vesicles, approximately 30-150 nm......

• • How Is Protein Extraction Performed from FFPE Tissue for Mass Spectrometry?

  Formalin-Fixed Paraffin-Embedded (FFPE) tissue is the most widely used method for sample preservation in clinical and basic research. Due to their stability during long-term storage and the wealth of associated clinical information, FFPE samples have become an essential resource in histology, pathology, and biomarker research. However, protein extraction from FFPE tissue for mass spectrometry remains a major technical challenge in proteomics studies. Challenges in Protein Extraction from FFPE Tissue ......