Resources

Proteomics Databases



Metabolomics Databases

• • Crosstalk between Lactylation and Other PTMs

  Lactate was once regarded as a metabolic by-product of glycolysis, but recent studies have fundamentally changed this understanding. In 2019, Zhang et al. first reported histone lactylation in Nature, demonstrating that lactate can participate in epigenetic regulation by covalently modifying lysine residues on histones. This discovery opened a new direction in the study of post-translational modifications (PTMs) and prompted extensive investigation into the synergistic and competitive mechanisms betwe......

• • What Is the Role of SIRT5 in Histone Malonylation?

  With the continued advancement of epigenetic regulation research, the study of histone post-translational modifications (PTMs) has expanded beyond classical acetylation and methylation to include a range of emerging lysine acylation modifications. Malonylation is one such modification that has attracted increasing attention in recent years and is closely associated with cellular metabolic status. As a predominantly mitochondria-localized deacylase, SIRT5 plays a critical role in regulating protein mal......

• • PhIP-Seq for Cohort Studies: Designing Case-Control Comparisons and Antibody Signature Discovery

  Learn when PhIP-Seq is the right choice for cohort-based antibody discovery, how to structure case-control comparisons, and how to define candidate antibody signatures with defensible analysis and validation planning.

• • How to Choose a Peptide Library for PhIP-Seq: Proteome-Wide, Pathogen-Focused, and Custom Designs

  Choose the right PhIP-Seq peptide library by matching study goals to proteome-wide, pathogen-focused, or custom designs.

• • Common PhIP-Seq Troubleshooting Questions: Weak Enrichment, Background Signals, and Library Coverage

  Learn how to troubleshoot weak enrichment, background signal, and uneven library coverage in PhIP-Seq data by separating assay setup issues from library performance, sequencing allocation, and analysis threshold problems.

• • Interpreting PhIP-Seq Results: Enriched Peptides, Cross-Reactivity, Controls, and Biological Relevance

  Technical guide for interpreting enriched peptides, controls, cross-reactivity, and validation readiness in phip seq analysis.

• • PhIP-Seq Applications in Autoantibody and Antigen Discovery: When the Method Fits the Research Question

  Technical guide for evaluating when PhIP-Seq fits autoantibody profiling, antigen discovery, and downstream validation planning.

• • Planning a PhIP-Seq Project: Sample Requirements, Controls, Library Choice, and Deliverables

  Technical guide for Planning a PhIP-Seq Project: Sample Requirements, Controls, Library Choice, and Deliverables.

• • PhIP-Seq vs Traditional Serology and Protein Arrays: Choosing the Right Antibody Profiling Method

  Compare PhIP-Seq, ELISA-style serology, and protein arrays for antibody profiling. Learn when to choose peptide-level discovery, targeted antigen testing, multiplex protein screening, or a staged validation workflow.

• • How to Quantify Histone Malonylation Levels?

  With the continuous advancement of metabolic epigenetics, research on histone post-translational modifications has progressively shifted from determining whether modifications occur to understanding how modification levels dynamically fluctuate under different biological conditions. Among these modifications, histone malonylation has emerged as a critical regulatory mechanism linking cellular metabolism to chromatin regulation, making it a major focus in the interdisciplinary fields of proteomics and ......