Resources
Proteomics Databases
Metabolomics Databases
-
Epitope screening is a critical technique for identifying epitopes—specific regions of an antigen molecule that are recognized by the immune system. These epitopes interact with antibodies or T-cell receptors, potentially triggering an immune response. The primary goal of epitope screening is to identify antigenic protein fragments capable of effectively activating the immune system, which is essential for optimizing vaccine design, selecting high-affinity antibodies, and investigating abnormal immune......
-
DDA mass spectrometry is a widely employed strategy for data acquisition in proteomics. Its core principle involves performing full-scan MS1 to obtain peptide ion information, followed by the automatic selection of the most intense precursor ions based on signal intensity for subsequent MS2 analysis. This approach enables the identification and quantification of proteins within complex biological samples. DDA mass spectrometry allows high-throughput protein identification, the generation of protein ........
-
• High Throughput Cell Screening
High throughput cell screening is a powerful technology that enables the rapid analysis of a large number of cell samples. It relies on automated and miniaturized processes to efficiently conduct cellular assays. The primary purpose of high throughput cell screening is to identify and analyze key biological characteristics of cells—such as proliferation, apoptosis, metabolic activity, and signal transduction pathways. These insights are critical for understanding cellular behaviors in various biologic......
-
• Protein Isoelectric Point Detection Techniques
Protein isoelectric point detection techniques are essential tools in proteomics, as the isoelectric point (pI) of a protein represents the pH at which it carries no net electrical charge in solution. At this specific pH, the positive and negative charges on the protein molecule are balanced, rendering the protein electrically neutral. The isoelectric point is a critical biochemical characteristic of a protein, influencing its solubility, structural conformation, biological function, and interactions ......
-
• How to Detect the Ubiquitination Levels of a Specific Protein
To Detect the ubiquitination levels of a protein is a crucial approach for understanding its stability, subcellular localization, and functional regulation. Ubiquitination is a type of post-translational modification that involves the covalent attachment of ubiquitin—a small regulatory protein—to a target protein. This modification can be detected using various techniques: Western Blotting Western blotting employs antibodies specific to both ubiquitin and the target protein to detect ubiquitination ......
-
To elucidate protein interaction networks, a variety of experimental techniques have been developed, among which the pull-down assay has emerged as one of the most extensively utilized approaches. This article presents a comprehensive examination of the pull-down assay, focusing on both its technical foundations and its biological relevance. Technical Principles The pull-down assay is grounded in the principle of affinity chromatography, leveraging the specific interactions between proteins to isola......
-
• Applications and Method Development of High-Resolution Mass Spectrometry in Proteomics
The application of high-resolution mass spectrometry in proteomics is primarily demonstrated by its ability to precisely measure the mass of proteins and peptides. This capability allows scientists to achieve highly sensitive and accurate identification of proteins and peptides, thereby playing a critical role in proteomics research. Principles and Technological Advancements of HRMS 1. Improvement of Mass Resolution HRMS enables the distinction of mass peaks that are extremely close in m/z values, w......
-
• Immunoprecipitation Combined with Mass Spectrometry Analysis
Immunoprecipitation combined with mass spectrometry analysis is a widely employed technique in proteomics that enables the identification of components within protein complexes and the characterization of protein–protein interactions. By leveraging the high specificity of immunoprecipitation and the high sensitivity of mass spectrometry, this approach facilitates the detection of proteins from complex biological samples. The workflow of immunoprecipitation combined with mass spectrometry analysis gene......
-
• Cryo-EM Single Particle Analysis in Structural Biology
Cryo-EM single particle analysis is an advanced structural biology technique that enables the determination of three-dimensional structures of biological macromolecules in a near-native state. This method involves rapidly vitrifying samples at cryogenic temperatures to preserve their natural conformation in an amorphous, non-crystalline form, allowing direct visualization of individual molecules without the need for staining or crystallization. Using large datasets of molecular images, researchers app......
-
Crystallography analysis is a critical technique for investigating the internal structure and atomic arrangement of materials. It plays a pivotal role across a range of disciplines, including chemistry, materials science, life sciences, and pharmaceutical development. By applying crystallography methods, researchers can determine crystal structures, bond lengths, bond angles, and intermolecular interactions with high precision. This structural insight is essential for understanding material properties......
How to order?
