Resources

Proteomics Databases



Metabolomics Databases

• • What Is CUT&Tag? Principles, Workflow, and Advantages Explained

  In recent years, the exploration of chromatin states has transitioned from ChIP-seq to more sensitive and efficient approaches. Among these, Cleavage Under Targets and Tagmentation (CUT&Tag) has gained significant prominence due to its high sensitivity, low background noise, and compatibility with high-throughput applications, making it a preferred technique in the field of chromatin epigenetics. This review provides a comprehensive examination of the principles, experimental workflow, and advantages of....

• • What Is Non‑Degradative Ubiquitination?

  In eukaryotic cells, ubiquitination represents a pivotal post-translational modification whereby the small protein ubiquitin is covalently conjugated to substrate proteins, thereby modulating their stability, activity, subcellular localization, and interaction networks. While the canonical and most extensively characterized role of ubiquitination is to direct proteins toward proteasomal degradation, typically via K48-linked polyubiquitin chains in the 26S proteasome pathway, accumulating evidence has ......

• • How Different Enrichment Strategies Impact Acetylated Protein Detection: A Comparative Review

  Protein acetylation, a crucial post-translational modification in eukaryotes, plays a pivotal role in regulating diverse biological processes, including gene expression, metabolic homeostasis, and cell cycle progression. In recent years, advances in mass spectrometry have significantly deepened acetylome research. However, because acetylation events often occur at low abundance, direct detection by mass spectrometry is frequently hindered by signal suppression from high-abundance proteins. Consequently.....

• • How to Enrich Acetylated Peptides Before Mass Spectrometry?

  Protein acetylation, predominantly occurring on lysine residues (K), represents a critical post-translational modification involved in regulating protein activity, stability, and subcellular localization. Owing to its inherently low abundance, weak signal intensity, and the complex background of proteomic samples, selective enrichment of acetylated peptides is essential prior to liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis to enhance detection sensitivity and achieve comprehensive.....

• • How to Use Acetylation Data for Pathway Enrichment Analysis in Research?

  Acetylation is a critical protein post-translational modification (PTM) that plays essential roles in diverse biological processes, including the regulation of cellular metabolism, signal transduction, and chromatin remodeling. With the advancement of high-resolution mass spectrometry technologies, researchers can systematically detect and identify acetylation sites, thereby enabling in-depth investigation of their biological functions. Within the vast datasets generated from acetylation studies, pathway...

• • How to Improve Sensitivity and Specificity in Acetylated Protein Quantification: Techniques and Reagents

  Acetylation, a pivotal post-translational modification (PTM), plays a critical role in chromatin remodeling, metabolic regulation, and signal transduction. With the continuous advancement of mass spectrometry-based proteomics, investigations into acetylated proteomes have deepened considerably. However, owing to the inherently low abundance, transient nature, and dynamic regulation of acetylation, improving the sensitivity and specificity of acetylated protein quantification analyses remains a central .....

• • What Is O-Glycoproteomics?

  O-glycoproteomics is a systematic field devoted to the comprehensive study of O-linked glycosylation modifications in proteins, encompassing the identification of modification sites, elucidation of glycan structures, and quantitative analysis. As one of the key areas in post-translational modification research, O-glycoproteomics is of great significance in elucidating cellular signal regulation, understanding disease mechanisms, and facilitating biomarker discovery. Basic Principles of O-Glycosylation .....

• • What is Quantitative Acetylproteomics? A Comprehensive Overview of Underlying Principles and Application Prospects

  Post-translational modification (PTM) represents one of the core mechanisms regulating protein function, and lysine acetylation has emerged as one of the most intensively studied PTM types following phosphorylation. Acetylation is involved in diverse biological processes, including transcriptional regulation, metabolic control, cell cycle progression, and signal transduction. Aberrant acetylation patterns are closely associated with various diseases, such as cancer, metabolic syndrome, and .......

• • How Mass Spectrometry Enables High-Precision Quantification of Phosphorylated Proteins?

  Protein phosphorylation is among the most prevalent and functionally significant post-translational modifications, playing a pivotal role in diverse biological processes such as cell signaling, proliferation, differentiation, and apoptosis. However, phosphorylation sites typically exhibit low abundance, a wide dynamic range, and susceptibility to enzymatic degradation, all of which pose significant challenges for high-precision quantification of phosphorylated proteins. Mass spectrometry (MS), as the ......

• • How Protein Lactylation Affects Gene Expression: Mechanistic Insights

  Research on protein post-translational modifications (PTMs) continues to reveal the intricate complexity of cellular regulation. Among these, protein lactylation has emerged as an important new focus in epigenetic studies. As a novel lysine modification, in addition to acetylation and methylation, lactylation not only participates in the metabolism–transcription regulatory network but may also play critical roles in diverse biological processes, including tumorigenesis, immune regulation, and stem cell ....