Eukaryotic Non-Ginseng Transcriptome Sequencing Service

Eukaryotic transcriptome sequencing can be divided into reference-based and non-reference-based scenarios. Among these, non-reference-based sequencing refers to analyzing the eukaryotic transcriptome without a reference genome. For eukaryotic reference-free sequencing, the raw data must first undergo quality control and be assembled into unigenes. These unigenes are then used as reference sequences for subsequent analyses, including functional annotation, SNP, and SSR marker development. Additionally, differential gene expression analysis and differential gene functional enrichment analysis can be performed on multiple samples to discover functional genes, thereby providing guidance for future research.

 

MtoZ Biolabs offers transcriptome sequencing services based on the high-throughput Illumina sequencing platform, which can detect the overall transcription level of any species at the single nucleotide level. This service not only analyzes the structure and expression levels of transcripts but also identifies unknown and rare transcripts. Furthermore, it accurately detects alternative splicing sites and coding single nucleotide polymorphisms (cSNP), thus delivering the most comprehensive transcriptome information.

 

Analysis Workflow

 



Eukaryotic Reference-Free Transcriptome Sequencing Workflow

 

Service Advantages

1. Experienced technicians provide a full range of professional services, from experimental design and sample testing to data analysis.

2. Clear and efficient processes reduce unnecessary sample and time waste, ensuring short delivery times.

3. MtoZ Biolabs has an independent bioinformatics analysis platform that offers not only routine analysis but also advanced custom analysis.

4. MtoZ Biolabs' multi-omics analysis platform, including proteomics and metabolomics, enables integrated multi-omics analysis, enhancing the quality of publications.

 

Sample Submission Requirements

1. Cells (≥5×10⁶), Fresh Plant Tissue (≥500 mg), Animal Tissue (≥300 mg), Blood (≥2 ml), FFPE Samples (≥10 Unstained HE Sections)

2. RNA Samples (Nucleic Acid Quality ≥3 μg, 260/280 ≥2.0, RIN Value ≥7, 28S/18S ≥1.0, Concentration ≥50 ng/μl, Volume ≥10 μl)

 

Case Study

 

MtoZ Biolabs Transcriptome Sequencing Case Study