Whole Transcriptome Sequencing Analysis Service

Whole transcriptome sequencing (WTS) provides a comprehensive analysis of the complete set of transcripts from specific tissues or cells under defined conditions. Utilizing next-generation sequencing (NGS) technology, WTS combines ribosomal RNA (rRNA) depletion, strand-specific library construction, and small fragment enrichment techniques to enable the sequencing and analysis of both coding RNA and non-coding RNA (miRNA, lncRNA, circRNA). This approach not only allows for the quantification of gene expression changes but also enables the investigation of non-coding RNA roles in regulating gene expression and the construction of ceRNA regulatory networks. WTS provides in-depth insights into transcriptional regulation, offering a powerful tool for decoding complex biological phenomena.

 

MtoZ Biolabs offers Whole Transcriptome Sequencing Analysis Service using cutting-edge sequencing platforms and comprehensive bioinformatics analysis. Our service delivers high-resolution, accurate transcriptome data that support a wide range of applications, including biomedical research, disease mechanism exploration, and drug development.

 

Analysis Workflow

 



 

Service Advantages

1. Comprehensive Data

Obtain complete information on four types of RNA (mRNA, lncRNA, miRNA, circRNA) from a single sample.

 

2. Advanced Correlation Analysis

Supports pairwise correlation analysis and ceRNA network construction, providing insights into regulatory mechanisms behind biological processes and narrowing down candidate gene selection.

 

3. High Sensitivity and Precision

Accurately detects low-abundance transcripts, making it ideal for complex biological samples.

 

4. In-depth Bioinformatics Analysis

Constructs regulatory networks and provides a detailed gene regulation map, uncovering potential regulatory mechanisms.

 

Sample Submission Requirements

(1) Cells ( ≥ 5×10⁶), fresh plant tissue ( ≥ 500 mg), animal tissue ( ≥ 300 mg), blood ( ≥ 2 mL), FFPE samples ( ≥ 10 sections without HE staining).

 

(2) RNA samples (nucleic acid quality ≥ 3 μg, 260/280 ≥ 2.0, RIN value ≥ 7, 28S/18S ≥ 1.0, concentration ≥ 50 ng/μL, volume ≥ 10 μL).

 

Applications

• Agriculture and Forestry: Study mechanisms of stress resistance, growth, development, and breeding research.
• Microbiology: Explore pathogen-host interactions, drug resistance mechanisms, and pathogenic mechanisms.
• Marine and Aquaculture: Apply in fisheries resource assessment, aquaculture, environmental monitoring, and food safety.
• Basic Medicine and Clinical Diagnostics: Contribute to biomarker discovery, disease mechanism exploration, disease classification, and personalized treatment.

 

MtoZ Biolabs offers Whole Transcriptome Sequencing Analysis Service, delivering high-precision sequencing data and comprehensive bioinformatics analysis to support researchers in exploring transcriptome dynamics and regulatory mechanisms across various biological systems. Free project evaluation, welcome to learn more details!