Untargeted Metabolomics Service
Untargeted metabolomics is a study in which all detectable metabolites are identified and quantitated for analyzing the overall metabolic characteristics and discovering metabolic mechanisms behind the phenomenon. Also known as discovery metabolomics, untargeted metabolomics is a powerful tool for large-scale early phase diagnosis and discovery of new biomarkers. Compared to traditional metabolic quantitation methods, untargeted metabolomics is a high-throughput technology, enabling more comprehensive analysis with more accurate data acquisition, eliminating biased research direction.
MtoZ Biolabs offers untargeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms.
Untargeted Metabolomics
MtoZ Biolabs is proud to offer high-efficient and accurate plant metabolomics analysis service, based on Thermo Q Exactive and AB Q-TOF 5600 mass spectrometry systems.
MtoZ Biolabs develops suitable microbial sample preparation and metabolites extraction strategies according to your projects, and assures high-efficient and accurate microbial metabolomics analysis.
Analytical Steps of Targeted Metabolomics
1. Data Collection
All detectable metabolites in both control and experimental groups are analyzed by corresponding mass spectrometry methods for discovering metabolites differential expression levels.
2. Identification of Differential Metabolites Through Metabolic Spectrum Analysis
3. Statistical Analysis and Bioinformatics Explanation to Uncover the Correlation Between the Metabolites and Metabolic Pathways or Reaction Processes Through ANOVA, PCA, KEGG Annotation Analyses, etc.
Analysis Workflow
Service Advantages
1. Standard Technical Platform
2. State-of-the-Art LC-MS (MRM) and GC-MS (SIM) Analysis Systems
3. Comprehensive Commercial Databases System
4. Professional Support and Precise Custom Service
Sample Types
Sample Submission Requirements
1. Cells and Microbes Samples
Cellular activities should be terminated instantly, whereas the cells remain intact.
2. Animal Body Fluids Samples
Such as urine, blood, saliva, etc. Samples must be added with anticoagulant and preservative reagents right after sample collection, followed by freezing at -80 °C.
3. Plant Tissues
200 mg/sample. Samples should be frozen in liquid nitrogen right after sample collection, and then transferred to -80 °C for storage.
4. Serum Samples
Repeated freezing and thawing of sample must be eliminated. Serum samples should be settled down at room temperature for 30 min in the collection tube, and then transferred to centrifuge tube and centrifuged at 8,000 rpm for 5 min. After centrifugation, supernatant is aliquoted to freezing tubes with 500 uL/sample, and stored at -80 °C.
5. Urine Samples
1 mL/sample. Urine samples can be aliquoted to centrifuge tubes with 1 mL each tube and addition of 1/100 (w/v) sodium azide, and stored at -80 °C.
6. Faeces samples
10 mg/sample
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. MS Data Quality Checks
5. Metabolites Quantification Data
6. Bioinformatics Analysis (PCA, KEGG, etc)
Related Services
Targeted Metabolomics
Untargeted Metabolomics
Lipidomics
Label-Free
iTRAQ/TMT
SILAC/Dimethyl
SWATH
MRM
Protein Identification
Protein Mass Measurement
PTMs Identification
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Human blood, as an important fluid sample in metabolomics, is mainly divided into plasma and serum. Compared to plasma, serum does not contain fibrinogen, and its components are relatively simple with over 1000 kinds of endogenous small molecule metabolites, making it an important metabolomics fluid sample. Due to the variety and extensive range of polarities of small molecule metabolites in serum, including highly polar substances such as amino acids, glucose, and nucleosides, as well as low-polarity......
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• Urine Metabolomics Analysis Service
Urine contains a vast array of chemicals, including microbial metabolites and mammalian metabolic products. Drugs, pollutants, and industrial chemicals may also be present in urine. Since urine lacks homeostatic regulation, it can reflect metabolic disturbances, providing insights into systemic changes in response to physiological challenges or disease progression.
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• Body Fluid Metabolomics Analysis Service
Body fluid metabolomics is an important branch of metabolomics. It focuses on animal body fluids, evaluating the impact of endogenous stimuli (such as drugs or toxins) on the body by qualitatively and quantitatively analyzing changes in endogenous metabolites within biological systems. Human metabolism involves a series of complex biophysical and biochemical reactions. The volume, electrolyte concentration, osmotic pressure, and pH of intracellular and extracellular fluids continuously influence cellu......
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• Exosome Untargeted Metabolomics Analysis Service
Exosome (EX) is a heterogeneous group of extracellular vesicle (EV) with size similar to viruses (30-150 nm). Many different cell types, including red blood cells, B cells, T cells, mast cells, platelets, endothelial cells, fibroblasts, adipocytes, epithelial cells, muscle, dendritic cells, and tumor cells, can release EX. The heterogeneous vesicle is exclusively formed by the fusion of multivesicular bady formed by the inward budding of the endosomal membrane with the plasma membrane, which are then ......
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• Cerebrospinal Fluid Untargeted Metabolomics Analysis Service
Cerebrospinal fluid (CSF) is secreted within the central nervous system (CNS) and fills in the ventricles, the subarachnoid space of the brain, and the spinal column. CSF's role is to protect the brain from physical impacts. Furthermore, CSF plays a crucial role in the circulation of nutrients and chemicals in the blood and in waste management by actively transporting or facilitating bulk flow (from the extracellular fluid of the brain to the subarachnoid space
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MtoZ Biolabs offers high-efficient and accurate plant metabolomics analysis service to meet your research needs.
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• Microbial Metabolomics Service
MtoZ Biolabs offers high-efficient and accurate microbial metabolomics analysis service to meet your specific needs.
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