SWATH Based Protein Quantitative Service

    Sequential Window Acquisition of all Theoretical fragment ions (SWATH) is a brand new mass spectrometry technology co-developed by Dr. Ruedi Aebersold research group (ETH Zürich) and AB SCIEX. SWATH data is an independent acquisition (DIA) technique. Unlike data dependent acquisition technique, which only selects certain MS spectrum for MS/MS detection, SWATH analyzes all MS and MS/MS spectra, allowing detection of virtually all detectable proteins in a sample. In SWATH technology, MS spectra are divided into several narrow windows and peptides in each window are sequentially analyzed equally, followed by MS/MS analysis. SWATH technique combines the high-throughput quality of shotgun proteomics with high accuracy comparable to MRM. To better meet our clients’ needs, MtoZ Biolabs offers a comprehensive SWATH service, including optimized sample preparation, high-quality SWATH mass spectrometry analysis and professional bioinformatics analysis.

     

    Analytical Platform

    AB SCIEX Triple-TOF 5600-plus

     

    Analysis Workflow

     

    image-SWATH-workflow2.png

     

    Service Advantages 

    1. Quantitatively Analyze Nearly All Detectable Molecules, Including Low Abundance Proteins and Peptides

    2. High Accuracy, Comparable to MRM Level

    3. High-Throughput, Identify and Quantitate More than 2000 Proteins at Once

    4. Complete Digital Archive of Sample in the First Analysis

    5. Pre-Experiment Method Development is Unnecessary

     

    Sample Submission Requirements 

     

    image-SWATH-Sample Require2.png

     

    Bioinformatics Analysis

    1. SWATH Data Quality Assessment

    2. Multivariate PCA Analysis

    3. Protein Statistical Analysis: Venn Diagram, Volcano Plot

    4. Functional Annotation: GO Annotation, KEGG Annotation, COG Annotation

    5. Clustering Analysis: Hierarchical Clustering, K-Means Clustering

    6. Network Analysis: STRING Analysis

     

    Deliverables

    1. Experiment Procedures

    2. Parameters of Liquid Chromatography and Mass Spectrometer

    3. MS Raw Data Files

    4. Peptide Identifications and Intensities

    5. Protein Identifications and Intensities

    6. Bioinformatics Analysis

     

    Related Services

    Quantitative Proteomics

    Label-Free

    iTRAQ/TMT

    SILAC/Dimethyl

    SWATH

    MRM

     

    Protein Analysis

    Protein Identification

    Protein Mass Measurement

    PTMs Identification

     

    PTM Analysis

    Phospho-Proteomics

    Acetyl-Proteomics

    Ubiquitin-Proteomics

    Glyco-Proteomics

    Disulfide Bond

    Histone Modifications

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