Label-Free
Overview
Label-free analysis is a powerful technique for identifying and quantifying relative changes in complex protein samples. In this method, samples are run individually and identified by MS/MS information. Relative quantitation of different samples is then analyzed by aligning chromatographic peak areas of precursor ions between various runs. The most distinctive advantage is that it quantifies proteins without any use of labels, and can analyze unlimited number of samples. Since Label-free is relatively simple and easy compared to other proteomics techniques, it has been widely used for proteomics study and biomarker discovery. MtoZ Biolabs is proud to offer Label-free quantitative proteomics service with faster simpler results. We can also perform MS3 analysis to ensure higher analytical accuracy of qualification and quantitation.
Service Workflow
Key Features
• Isotopic labels are not required
• Simpler experimental procedures and data analysis
• Samples are run individually, and numbers of samples are unlimited
• Suitable for analyzing various types of samples, including tissue, cell, blood, etc.
Sample Requirements
*Note: For Co-IP protein samples, only 2-5 ug is required. During sample elution, detergents (such as SDS) and high oxygen concentration should be avoided. Alternatively, you can send the sample beads to us directly.
Bioinformatics Analysis
• Label-free MS data quality assessment
• Multivariate PCA analysis
• Protein statistical analysis: Venn Diagram, Volcano Plot
• Functional annotation: GO annotation, KEGG annotation, COG annotation
• Clustering analysis: Hierarchical clustering, K-means clustering
• Network analysis: STRING analysis
Reports
• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensities
• Protein identifications and intensities
• Bioinformatics analysis
Related Services
Label-Free
iTRAQ/TMT
SILAC/Dimethyl
SWATH
MRM
Protein Identification
Protein Mass Measurement
PTMs Identification
Phospho-proteomics
Acetyl-proteomics
Ubiquitin-proteomics
Glyco-proteomics
Disulfide bond
Histone Modifications
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