Label-Free Quantitative Proteomics Service, MS Based

    Label-free analysis is a powerful technique for identifying and quantifying relative changes in complex protein samples. In this method, samples are run individually and identified by MS/MS information. Relative quantitation of different samples is then analyzed by aligning chromatographic peak areas of precursor ions between various runs. The most distinctive advantage is that it quantifies proteins without any use of labels, and can analyze unlimited number of samples. Since Label-free is relatively simple and easy compared to other proteomics techniques, it has been widely used for proteomics study and biomarker discovery. MtoZ Biolabs is proud to offer Label-free quantitative proteomics service with faster simpler results. We can also perform MS3 analysis to ensure higher analytical accuracy of qualification and quantitation.

     

    Analysis Workflow

     

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    Service Advantages

    1. Isotopic Labels are Not Required

    2. Simpler Experimental Procedures and Data Analysis

    3. Samples are Run Individually, and Numbers of Samples are Unlimited

    4. Suitable for Analyzing Various Types of Samples, Including Tissue, Cell, Blood, etc.

     

    Sample Submission Requirements

     

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    *Note: For Co-IP protein samples, only 2-5 ug is required. During sample elution, detergents (such as SDS) and high oxygen concentration should be avoided. Alternatively, you can send the sample beads to us directly.

     

    Bioinformatics Analysis

    1. Label-Free MS Data Quality Assessment

    2. Multivariate PCA Analysis

    3. Protein Statistical Analysis: Venn Diagram, Volcano Plot

    4. Functional Annotation: GO Annotation, KEGG Annotation, COG Annotation

    5. Clustering Analysis: Hierarchical Clustering, K-Means Clustering

    6. Network Analysis: STRING Analysis

     

    Deliverables

    1. Experiment Procedures

    2. Parameters of Liquid Chromatography and Mass Spectrometer

    3. MS Raw Data Files

    4. Peptide Identifications and Intensities

    5. Protein Identifications and Intensities

    6. Bioinformatics Analysis

     

    Related Services

    Quantitative Proteomics

    Label-Free

    iTRAQ/TMT

    SILAC/Dimethyl

    SWATH

    MRM

     

    Protein Analysis

    Protein Identification

    Protein Mass Measurement

    PTMs Identification

     

    PTM Analysis

    Phospho-Proteomics

    Acetyl-Proteomics

    Ubiquitin-Proteomics

    Glyco-Proteomics

    Disulfide Bond

    Histone Modifications

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