Ubiquitin-proteomics

Overview

Ubiquitination is an important PTM in cell biology, as the ubiquitin-proteasome complex has mediated degradation of 80-85 percent proteins, and directly affects the expression level and function of proteins. MtoZ Biolabs is glad to offer ubiquitination identification and quantification service through high-resolution mass spectrometry analysis. For some low abundance ubiquitin-proteins, we can provide peptide enrichment service before mass spectrometry analysis. To ensure high specificity and efficacy of enrichment of ubiquitin-peptides, we use CST Ubiquitin antibody for enrichment process, and use 2-3 different enzymes for protein digestion to ensure full scan of ubiquitin-peptides.

MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for ubiquitin-proteomics analysis service. To better solve your research problems, we offer an all-inclusive service, including protein purification, digestion, peptide enrichment and separation, LC-MS/MS analysis, MS data analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell/protein samples, and we will complete all the following experiments.



Service Workflow



Key Features

• High-throughput analysis, identify and quantify up thousands of proteins at once
• Quantitatively analyze low abundance ubiquitin-proteins
• Use 2-3 enzymes for protein digestion, ensure full-coverage analysis
• State-of-art Nano LC and Orbitrap Fusion Mass Spectrometer

Sample Requirement



*Note: We will perform testing experiments before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.

Reports

• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensities
• Protein identifications and ubiquitin mapping
• Bioinformatics analysis

Related Services

PTM Analysis

Phospho-proteomics
Acetyl-proteomics
Ubiquitin-proteomics
Glyco-proteomics
Disulfide bond
Histone Modifications



Protein Analysis

Protein Identification
Protein Mass Measurement
PTMs Identification



Protein Sequencing

Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-length Sequencing