Quantitative Ubiquitinomics Service

    Ubiquitination is an important PTM in cell biology, as the ubiquitin-proteasome complex has mediated degradation of 80-85 percent proteins, and directly affects the expression level and function of proteins. MtoZ Biolabs is glad to offer ubiquitination identification and quantification service through high-resolution mass spectrometry analysis. For some low abundance ubiquitin-proteins, we can provide peptide enrichment service before mass spectrometry analysis. To ensure high specificity and efficacy of enrichment of ubiquitin-peptides, we use CST Ubiquitin antibody for enrichment process, and use 2-3 different enzymes for protein digestion to ensure full scan of ubiquitin-peptides.

    MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for ubiquitin-proteomics analysis service. To better solve your research problems, we offer an all-inclusive service, including protein purification, digestion, peptide enrichment and separation, LC-MS/MS analysis, MS data analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell/protein samples, and we will complete all the following experiments.

    Analysis Workflow

    image-Ubiquitin-workflow2.png

     

    Service Advantages

    1. High-Throughput Analysis, Identify and Quantify Up Thousands of Proteins at Once
    2. Quantitatively Analyze Low Abundance Ubiquitin-Proteins
    3. Use 2-3 Enzymes for Protein Digestion, Ensure Full-Coverage Analysis
    4. State-of-Art Nano LC and Orbitrap Fusion Mass Spectrometer

     

    Sample Submission Requirements

     
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    *Note: We will perform testing experiments before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.

     

    Deliverables

    1. Experiment Procedures
    2. Parameters of Liquid Chromatography and Mass Spectrometer
    3. MS Raw Data Files
    4. Peptide Identifications and Intensities
    5. Protein Identifications and Ubiquitin Mapping
    6. Bioinformatics Analysis

     

    Related Services

    PTM Analysis

    Phospho Proteomics
    Acetyl Proteomics
    Ubiquitin Proteomics
    Glyco Proteomics
    Disulfide Bond
    Histone Modifications

    Protein Analysis

    Protein Identification
    Protein Mass Measurement
    PTMs Identification

    Protein Sequencing

    Protein De Novo Seq
    N-Terminal Sequencing
    C-Terminal Sequencing
    Edman Degradation
    Protein Full-Length Sequencing

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