Quantitative Ubiquitinomics Service

Ubiquitination is an important PTM in cell biology, as the ubiquitin-proteasome complex has mediated degradation of 80-85 percent proteins, and directly affects the expression level and function of proteins. MtoZ Biolabs is glad to offer ubiquitination identification and quantification service through high-resolution mass spectrometry analysis. For some low abundance ubiquitin-proteins, we can provide peptide enrichment service before mass spectrometry analysis. To ensure high specificity and efficacy of enrichment of ubiquitin-peptides, we use CST Ubiquitin antibody for enrichment process, and use 2-3 different enzymes for protein digestion to ensure full scan of ubiquitin-peptides.

MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for ubiquitin-proteomics analysis service. To better solve your research problems, we offer an all-inclusive service, including protein purification, digestion, peptide enrichment and separation, LC-MS/MS analysis, MS data analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell/protein samples, and we will complete all the following experiments.



Analysis Workflow



 

Service Advantages

1. High-Throughput Analysis, Identify and Quantify Up Thousands of Proteins at Once
2. Quantitatively Analyze Low Abundance Ubiquitin-Proteins
3. Use 2-3 Enzymes for Protein Digestion, Ensure Full-Coverage Analysis
4. State-of-Art Nano LC and Orbitrap Fusion Mass Spectrometer

 

Sample Submission Requirements

 


*Note: We will perform testing experiments before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.

 

Deliverables

1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Peptide Identifications and Intensities
5. Protein Identifications and Ubiquitin Mapping
6. Bioinformatics Analysis

 

Related Services

PTM Analysis

Phospho Proteomics
Acetyl Proteomics
Ubiquitin Proteomics
Glyco Proteomics
Disulfide Bond
Histone Modifications



Protein Analysis

Protein Identification
Protein Mass Measurement
PTMs Identification



Protein Sequencing

Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-Length Sequencing