Protein Full-length Sequencing
Overview
Full-length sequence information is essential for studying the integrity and correct translation of biopharmaceuticals. In general, protein sequence analysis service provided by MtoZ Biolabs includes three steps: digestion of proteins into smaller peptide fragments; tandem LC-MS/MS analysis of peptides; MS/MS data analysis. Traditional protein sequencing only uses trypsin for digestion, which renders the final protein sequence coverage of around 60%. To ensure 100% protein sequence coverage, MtoZ Biolabs uses up to 6 kinds of protein enzymes for protein digestions, eliminating any missing peptides.
Service Workflow
Applications
• Determination of full-length sequence of Proteins/Peptides/Antibodies/Vaccines
• Analysis of PTMs & chemical modifications on protein
• Verification of the correct translation of Recombinant Proteins
Sample Requirements
| Format | Gel bands, gel spots, and liquid samples are acceptable. |
| Quantity | Gel bands/spots that are visible to naked eyes are adequate for protein identification. For liquid samples, a total of 5-10 ug proteins are required. |
| Purity | Purity of liquid samples should be as high as possible. For liquid sample <10 ug, please avoid large amounts of detergent and salt ions, and note the buffer composition and the estimated amount of total proteins when submitting samples. |
| Note | All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated. |
Case Study
In this project, we analyzed the full-length sequence of an antibody product sent from one of our customers. To ensure 100% sequence coverage, we selected 4 enzymes for protein digestion, based on the predicted antibody sequence. The sequence mapping results showed 100% sequence coverage.
Reports
• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensity
• Protein identifications and intensity
Related Services
Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-length Sequencing
Protein Identification
Protein Mass Measurement
PTMs Identification
Phospho-proteomics
Acetyl-proteomics
Ubiquitin-proteomics
Glyco-proteomics
Disulfide bond
Histone Modifications
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