De Novo Protein Sequencing Service

    Protein sequences are critical information for study of protein function, as the amino acid sequence determines the advanced protein structures. Generally, there are two methods for protein sequence analysis, database search and de novo sequencing. Database search method identifies proteins by measuring peptide mass, and comparing to available protein databases. However, this method is unsuitable for analyzing antibodies, peptides, and proteins, of which the sequence information is either inaccessible, or not included in any databases. For these cases, de novo sequencing is the only way to decipher the protein sequence. MtoZ Biolabs uses the most advanced mass spectrometer-Orbitrap Fusion Lumos, for de novo sequencing service. Coupled with our professional bioinformatics analysis and reverse sequence confirmation, high-quality sequence analysis is guaranteed. Our de novo sequencing service is also well-suited for analyzing multiple kinds of antibodies, including IgM and IgG antibody subtypes, and antibodies modified with FITC, biotin, and Alexa, etc.

     

    Analysis Workflow 

     

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    Applications

    1. Sequence Analysis of Protein/Peptide, of which Databases are Unavailable

    2. PTMs Analysis of Protein/Peptide, of which Databases are Unavailable

    3. Sequence Analysis of CDR Region or Full-Length of Antibodies

     

    Case Study

    In this study, the full-length sequence of a monoclonal antibody is analyzed by our de novo sequencing system. MS/MS data in Fig. 1 indicated that complete fragmentation of peptide precursors and L/I residues can be easily discriminated after second collision. We used four enzymes (indicated by different color in Fig. 2) for protein digestion. Peptide mapping results showed complete sequence coverage with CDR being identified by at least 10 times, reaffirming accuracy of our sequence analysis.

     

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    Deliverables

    1. Experiment Procedures

    2. Parameters of Liquid Chromatography and Mass Spectrometer

    3. MS Raw Data Files

    4. Peptide Identifications, Intensity, and Peptide Mapping

    5. Protein Sequence and PTMs Data

     

    Related Services

    Protein Sequencing

    Protein De Novo Seq

    N-Terminal Sequencing

    C-Terminal Sequencing

    Edman Degradation

    Protein Full-Length Sequencing

     

    Protein Analysis

    Protein Identification

    Protein Mass Measurement

    PTMs Identification

     

    PTM Analysis

    Phospho-Proteomics

    Acetyl-Proteomics

    Ubiquitin-Proteomics

    Glyco-Proteomics

    Disulfide Bond

    Histone Modifications

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