iTRAQ/TMT/MultiNotch Quantitative Proteomics Service

    Isobaric Tag for Relative Absolute Quantitation (iTRAQ) and Tandem Mass Tags (TMT) are two similar quantitative proteomic techniques developed by AB SCIEX and Thermo Fisher, respectively. The most distinctive advantage of these two techniques is that they can label up to 10 different samples and compare the relative proteomic change through LC-MS/MS analysis. Therefore, these techniques are widely applied to study proteomic changes under different treatments or in different development stages. MtoZ Biolabs is proud to offer iTRAQ/TMT analysis service to meet your research needs. On the basis of these two well-established techniques, we can also provide MultiNotch MS analysis service through MS3 or proton-transfer reactions (PTR) to further increase the accuracy of the proteomic quantitation. MtoZ Biolabs has developed a specialized platform, equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, Orbitrap Fusion Lumos mass spectrometers coupled with Nano-LC for iTRAQ/TMT analysis service. To better solve your research problems, we offer an all-inclusive service, including protein reduction and digestion, peptide labeling, LC-MS/MS analysis, MS data analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your protein samples, and we will complete all the following experiments.

     

    The iTRAQ/TMT isobaric tags are similar and generally consist of three parts, reporter group, balance group, and reactive group. Peptides are labeled with isobaric tags by reacting with the reactive group. In MS spectrum, different iTRAQ-tag labeled peptides have the same mass, and thus appear in the same MS peaks. However, when fragmented in MS/MS, the reporter group dissociates to produce ion signals, which provide quantitative information regarding the relative amount of the peptide in the samples.

     

    Analysis Workflow

     

    image-iTRAQ-TMT-workflow2.png

     

    Service Advantages

    1. High-Throughput: Analyze Up to 10 Samples at Once

    2. In Vitro Labeling: Compatible for Labeling Various Samples, Including Tissue, Cell, Blood, etc.

    3. High Sensitivity and Broad Detecting Range: Suitable for Analyzing Low Abundance Proteins

    4. Compatible with Peptide Enrichment Experiment for PTMs Analysis

     

    Applications

    Analysis of large quantity of clinical samples, large scale of experimental analysis, and discovery of new biomarkers

     

    Sample Submission Requirements

     

    image-iTRAQ-Sample Require2.png

     

    Bioinformatics Analysis

    1. iTRAQ/TMT Data Quality Assessment

    2. Multivariate PCA Analysis

    3. Protein Statistical Analysis: Venn Diagram, Volcano Plot

    4. Functional Annotation: GO Annotation, KEGG Annotation, COG Annotation

    5. Clustering Analysis: Hierarchical Clustering, K-Means Clustering

    6. Network Analysis: STRING Analysis

     

    Deliverables

    1. Experiment Procedures

    2. Parameters of Liquid Chromatography and Mass Spectrometer

    3. MS Raw Data Files

    4. Peptide Identifications and Intensities

    5. Protein Identifications and Intensities

    6. Bioinformatics Analysis

     

    Related Services

    Quantitative Proteomics

    Label-Free

    iTRAQ/TMT

    SILAC/Dimethyl

    SWATH

    MRM

     

    Protein Analysis

    Protein Identification

    Protein Mass Measurement

    PTMs Identification

     

    PTM Analysis

    Phospho-proteomics

    Acetyl-proteomics

    Ubiquitin-proteomics

    Glyco-proteomics

    Disulfide bond

    Histone Modifications

Submit Inquiry
Name *
Email Address *
Phone Number
Inquiry Project
Project Description *

 

How to order?


/assets/images/icon/icon-message.png

Submit Inquiry

/assets/images/icon/icon-return.png