TMT Quantitative Proteomics Analysis Service

Tandem mass tags, TMT technology is a tandem mass spectrometry labeling technology developed by Thermo Fisher Company, mainly used for the identification and quantification of proteins in different samples. Each TMT labeling reagent consists of an NHS-ester group with amine-reactive activity, a mass normalization group, and an MS/MS reporter group. The isotope label of each labeling reagent has the same precursor mass. After the peptide segments are labeled by the reagent, they can react with the N-terminal and primary amine groups of the amino acid side chains and then be detected through the reporter group.

 

The TMT technology can use up to 11 stable isotope labels, allowing simultaneous comparison of the relative amounts of proteins in up to 10 different samples and can label almost all peptide or protein samples. Due to the ability of TMT labeling technology to be applicable to up to 11 samples, TMT is widely used in the discovery of quantitative protein biomarkers. By choosing 2-plex, 6-plex, or 10-plex isotope labels, the relative amounts of proteins in 2 groups, 6 groups, or 10 groups of different samples can be compared simultaneously.

 

TMT quantitative analysis is performed by measuring the intensity of the fragment reporter group ions released in tandem mass spectrometry mode (MS2) during peptide fragmentation. Precursor ions are selected for fragmentation in full scan mode (MS1), and the ion selection step reduces noise levels, providing favorable conditions for subsequent analysis steps. Ideally, only one selected precursor ion undergoes fragmentation during the precursor ion fragmentation process. However, in practice, other precursor ions are usually captured within the specified m/z window and fragmented together with the selected precursor.

 

MtoZ Biolabs uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nano-liquid chromatography to provide TMT-based proteomics analysis services. The Obitrap Fusion Lumos mass spectrometer is the highest resolution and sensitivity mass spectrometer currently available, ensuring the sensitivity for identifying low-abundance peptide fragments. Additionally, it employs a combination of HCD and ETD modes during peptide fragmentation, ensuring the integrity of peptide fragments.

 

**TMT proteomics analysis technology can be used for phosphoproteomics analysis.

 

Sample Submission Requirements

MtoZ Biolabs can perform TMT proteomics analysis on samples in both liquid and solid states. Solid samples can be transported with ice packs; liquid samples can be vacuum-dried or freeze-dried and transported with ice packs or transported with dry ice. Welcome to call for consultation.