Exosomal Small RNA/miRNA Sequencing Service

Exosomal Small RNA/miRNA Sequencing Service utilizes high-throughput sequencing technology to sequence miRNAs and other small RNAs within exosomes, enabling a full characterization of the roles of exosomal small RNAs in biological and pathological processes. Small RNAs are a class of short non-coding RNAs known to be present within exosomes, which can be transported between exosome-containing cells and absorbed by nearby or distant recipient cells. These exosomal small RNAs, particularly miRNAs, play crucial roles in various biological processes, including gene expression regulation and intercellular communication, as well as in the development and progression of diseases. Gaining comprehensive and accurate insights into exosomal miRNAs is essential for developing non-invasive diagnostic tools, improving patient prognosis, and facilitating therapeutic interventions.

Next-generation sequencing (NGS) is a practical and powerful method for studying and identifying exosomal small RNAs, offering a panoramic molecular profile and achieving comprehensive coverage of both known and novel small RNAs. Leveraging NGS technology, MtoZ Biolabs provides Exosomal Small RNA/miRNA Sequencing Service to enable thorough identification of exosome-derived small RNAs/miRNAs, supporting researchers in uncovering disease mechanisms, discovering novel biomarkers, and developing therapeutic targets.

 



Hu G. et al. Frontiers in Genetics. 2012.

 

Analysis Workflow

1. Exosome Isolation

Efficient extraction of high-purity exosomes from various sample types.

 

2. Small RNA Extraction

Isolation of high-quality small RNAs from exosomes using optimized protocols specifically designed for low-abundance RNA extraction.

 

3. Small RNA Library Construction

Construction of high-quality small RNA sequencing libraries using specially designed adapters tailored to small RNA characteristics, followed by ligation, reverse transcription, and amplification. Compatible with low-input RNA samples.

 

4. High-Throughput Sequencing

Comprehensive sequencing of known and novel miRNAs and other small RNA molecules using mainstream platforms such as Illumina.

 

5. Data Analysis and Reporting

Includes data quality control, identification of known and novel miRNAs, expression quantification, differential expression analysis, target gene prediction, functional enrichment, and regulatory network analysis. A detailed report is provided.

 

Applications

Some applications of Exosomal Small RNA/miRNA Sequencing Service:

Biomarker Discovery for Diseases

Systematic screening of exosomal small RNAs/miRNAs to identify non-invasive diagnostic and prognostic biomarkers for cancers, cardiovascular diseases, neurological disorders, and more.

 

Molecular Mechanism Studies

Uncovering the regulatory roles of exosomal small RNAs in processes such as cell-to-cell communication, inflammation, immune regulation, and metabolic control.

 

Drug Response and Resistance Mechanism Exploration

Analyzing dynamic changes in exosomal small RNAs before and after drug treatment to identify potential drug targets and drug resistance-associated miRNAs.

 

Exosome Engineering

Providing RNA profiling data to support exosome-based drug delivery and targeted delivery system development, facilitating the optimization of exosome carrier design.

 

Discovery of Novel Small RNA Molecules

Identifying unknown or unannotated novel small RNAs/miRNA-derived fragments to expand the understanding of exosome functions.

 

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Exosomal Small RNA/miRNA Sequencing Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Exosomal Small RNA/miRNA Sequencing Service data, providing clients with a comprehensive data report.

4. Comprehensive Coverage: Systematic analysis of both known and novel exosomal small RNAs/miRNAs to uncover complex regulatory networks.

5. Accurate Quantification: Sequencing-based exosomal small RNA data enable not only sequence identification but also precise quantification of RNA abundance.

 

FAQ

Q. What Sample Types are Compatible with Exosomal Small RNA/miRNA Sequencing?

Exosomal Small RNA/miRNA Sequencing Service is compatible with a variety of biological samples, including cell culture supernatants, plasma, urine, and other biological fluids known to contain exosomes.

 

Q. What does the Data Analysis Workflow for Exosomal Small RNA/miRNA Sequencing Service Include?

Our data analysis workflow comprehensively covers the entire process of exosomal small RNA/miRNA analysis, from sequence identification to functional interpretation. This includes data quality control, miRNA identification, expression quantification and differential analysis, target gene prediction, functional enrichment analysis, and pathway analysis.

 

Case Study

This study utilized small RNA high-throughput sequencing technology to systematically analyze the small RNA expression profiles in exosomes derived from the umbilical cord plasma of intrauterine growth restriction (IUGR) lambs. A large number of known and novel miRNAs were identified, and several miRNAs with significantly differential expression between IUGR and normal lambs were screened. Further target gene prediction and functional enrichment analysis revealed that these differential miRNAs primarily regulate key signaling pathways related to embryonic development, angiogenesis, cell proliferation, and nutrient metabolism. The findings highlight the potential regulatory role of exosomal miRNAs in the development of IUGR and provide new insights for molecular mechanism research and the development of potential biomarkers for IUGR.

 



Lu J. et al. Communications Biology. 2023.