Untargeted Proteomics Service

Untargeted proteomics is an advanced analytical technique that enables comprehensive and unbiased analysis of proteins in biological samples. Unlike traditional targeted proteomics methods, untargeted proteomics does not rely on specific protein labeling or target selection, offering a more comprehensive and detailed understanding of proteome complexity. By employing high-resolution liquid chromatography-mass spectrometry (LC-MS), this method detects unknown proteins, identifies low-abundance molecules, and quantifies changes in protein expression profiles, providing valuable insights for basic research, disease mechanisms, and drug development.

 

 

Tracz, J.et al. Int. J. Mol. Sci. 2021.

Figure 1. Characteristics of Targeted and Untargeted Proteomics

 

As a professional biotechnology service provider, MtoZ Biolabs is committed to offering high-quality Untargeted Proteomics Service to help researchers explore the untapped potential of the proteome. Our service is built on advanced technical platforms and supported by an experienced research team to meet various research needs and unlock the potential of life sciences.

 

Analysis Workflow

Our Untargeted Proteomics Service ensures the most comprehensive and reliable data through the following core steps:

1. Sample Preparation and Processing

We offer standardized and high-quality sample preparation workflows to ensure the optimal retention of biological information in every sample. This includes protein extraction, purification, and enrichment from cell, tissue, or body fluid samples.

 

2. Mass Spectrometry Analysis

We utilize state-of-the-art mass spectrometers, such as Q-Exactive and Orbitrap, to capture all protein molecules present in the sample using Data-Dependent Acquisition (DDA) or Data-Independent Acquisition (DIA) modes.

 

3. Data Analysis

Using professional bioinformatics tools and databases, we conduct thorough analysis of mass spectrometry data, including protein identification, quantification, functional annotation, and pathway analysis, to uncover potential biomarkers and key biological mechanisms.

 

4. Results Reporting and Interpretation

We not only provide detailed analytical reports but also offer in-depth biological interpretations and recommendations tailored to your needs, helping you understand the biological significance of the data.

 

Service Advantages

·  Multi-Technology Platforms: By integrating various mass spectrometry techniques (e.g., DDA, DIA, and labeling techniques), we are able to offer tailored untargeted proteomics analysis solutions that meet the needs of different samples and research objectives.

·  High Sensitivity and High Throughput: With advanced mass spectrometry instruments and efficient data processing platforms, we ensure high sensitivity, accuracy, and comprehensive results.

·  Expert Data Interpretation: In addition to routine protein identification, our expert team offers in-depth bioinformatics analysis, helping clients extract biological significance from the data and identify potential biomarkers, disease mechanisms, or therapeutic targets.

·  Comprehensive Technical Support: We provide full support from sample preparation, experimental design to data interpretation, ensuring that every study is conducted efficiently and accurately.

·  One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

Sample Submission Suggestions

Our Untargeted Proteomics Service can handle a variety of biological sample types, including but not limited to:

• Cell or Tissue Samples: From cell cultures, tissue sections, to whole blood or body fluid samples, all are suitable for analysis.
• Blood and Body Fluid Samples: Including serum, plasma, urine, saliva, etc., for disease biomarker screening and clinical research.
• Animal Models and Clinical Samples: We provide comprehensive analysis services for experimental animal and clinical samples to meet diverse research needs.

Note: Please ensure that an adequate sample amount is provided, as specific requirements may vary depending on the experiment type. Our technical support team will provide detailed sample preparation guidelines based on your sample type and research goals.

 

Applications

Our Untargeted Proteomics Service can address the following key issues:

• Discovering potential biomarkers for early diagnosis and disease monitoring.
• Identifying and quantifying protein expression patterns under different conditions, providing foundational data for understanding pathological mechanisms.
• Revealing protein interaction networks and their changes under various physiological states.
• Supporting target discovery and drug efficacy evaluation in new drug development.

 

Case Study

Case 1: Untargeted Proteomics Reveals the Effects of Schizophrenia and Drug Treatment on Adipose Tissue

This study employed untargeted proteomics coupled with high-resolution mass spectrometry, to analyze the effects of MAM (methylammonium carbamate) induced schizophrenia models and antipsychotic drug treatments on protein expression in adipose tissue. The results showed that both the MAM model and drug treatment significantly altered the expression levels of proteins related to metabolism, inflammation, and energy regulation in adipose tissue, revealing the potential systemic effects of schizophrenia and its treatment on metabolism. This study highlighted the crucial role of Untargeted Proteomics Service in complex disease mechanism research and provided important clues and potential targets for exploring systemic effects and optimizing treatment strategies for schizophrenia.

 



Kucera, J. et al. Prog. Neuropsychopharmacol. Biol. Psychiatry. 2020.

 

Case 2：Untargeted Proteomics in Plant Development Research

This study utilized HPLC-MS/MS to perform untargeted analysis of the proteome during early leaf development in Arabidopsis thaliana. The analysis revealed dynamic changes in protein expression and regulatory mechanisms. By analyzing protein expression profiles at different developmental stages, the study identified several key proteins related to cell proliferation, differentiation, and metabolism, and constructed a regulatory network for leaf development. This research demonstrated the significant potential of Untargeted Proteomics Service in revealing complex biological mechanisms, providing vital support for plant development research and molecular mechanism exploration.

 



Omidbakhshfard, M. A. et al. Patterns. 2021.

  

You will gain a deeper understanding of the proteome and uncover potential biomarkers, therapeutic targets, and disease mechanisms through MtoZ Biolabs' Untargeted Proteomics Service. We offer tailored solutions to accelerate your research and drive scientific breakthroughs. For any inquiries or further information, our expert team is here to assist you. Contact us today to explore how we can support your research.