DIA Quantitative Proteomics Analysis Service

DIA Quantitative Proteomics (Data-Independent Acquisition, DIA) is an advanced high-throughput mass spectrometry technique used for comprehensive and unbiased quantitative protein analysis. The basic principle involves dividing the entire mass spectrum scanning range into multiple small windows, within which all ions are simultaneously fragmented, and data is acquired using high-resolution mass spectrometry. This mode ensures that all detectable peptides and proteins are recorded, avoiding the loss of low-abundance proteins that often occurs in traditional DDA (Data-Dependent Acquisition) methods due to their data-dependency, thereby improving data reproducibility and coverage.

 

DIA quantitative proteomics analysis service is widely applied in various fields, including biomarker discovery, disease mechanism research, drug target screening, immunology and oncology studies, as well as in agriculture, environmental science, and food safety testing. Its high sensitivity, accuracy, and reproducibility make it an essential tool in fields such as precision medicine and protein interaction network research.

 



Pappireddi, N. et al. ChemBioChem, 2019.

Figure 1. DIA Mass Spectrometry Acquisition Principle.

 

Services at MtoZ Biolabs

Based on the AB SCIEX Triple-TOF 5600 plus high-resolution mass spectrometry system, which features high sensitivity, high resolution, high stability, and high speed, MtoZ Biolabs has established a DIA quantitative proteomics analysis service platform. You only need to send us your samples, and we will handle all subsequent steps of the project, including protein extraction, protein digestion, peptide separation, mass spectrometry analysis, raw data analysis, and bioinformatics analysis.

 

Service Advantages

1. Advanced Analysis Platform

MtoZ Biolabs established an advanced DIA quantitative proteomics analysis service platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all proteomics analysis data, providing clients with a comprehensive data report.

 

4. Customized Solutions

Customized experimental plans can be designed according to research needs (such as specific protein pathway analysis, post-translational modification analysis, protein interaction studies, etc.), catering to various application scenarios including basic research, disease research, and drug development.

 

Applications

1. Disease Research and Biomarker Screening

DIA quantitative proteomics analysis service can analyze the protein expression differences between healthy and diseased samples, identify disease-related biomarkers, and promote early diagnosis and precision treatment of major diseases such as cancer, cardiovascular diseases, and neurodegenerative diseases.

 

2. Drug Development and Mechanism of Action Research

In the process of new drug development, quantitative proteomics can be used for drug target screening, drug mechanism of action studies, as well as efficacy and toxicity assessments, assisting in the discovery and optimization of new drugs.

 

3. Cell Signaling Pathways and Function Research

DIA quantitative proteomics analysis service can be used to analyze the expression levels and post-translational modifications under different physiological or pathological conditions, revealing the dynamic changes in cell signaling pathways and helping to elucidate biological mechanisms.

 

4. Personalized Medicine and Precision Medicine

By combining proteomics data with multi-omics analysis, patient stratification can be achieved, supporting the development of personalized treatment plans, such as targeted therapies and immunotherapies for cancer.

 

5. Agriculture and Food Science

In agricultural research, DIA quantitative proteomics analysis service can be used for crop resistance studies, livestock and poultry health monitoring, and more. In the field of food science, it can be applied to food quality control, functional food ingredient identification, and more.

 

6. Biopharmaceutical Development and Quality Control

In the development of biopharmaceuticals such as antibody drugs, vaccines, and protein therapies, quantitative proteomics can be used for protein purity analysis, batch stability testing, and other quality control processes to ensure product safety and efficacy.

 

Case Study

1. Quantitative Proteomics Analysis of Serum and Urine Using DIA Mass Spectrometry

This study focuses on the proteomic characteristics of pediatric obstructive sleep apnea (OSA) and aims to identify potential biomarkers in serum and urine for early diagnosis and disease monitoring of OSA. The research utilized DIA quantitative proteomics analysis services to analyze serum and urine samples from pediatric OSA patients and compared them to healthy children to explore differences in protein expression. The study found significant differential expression of proteins in the serum and urine of OSA children, mainly involving inflammation, oxidative stress, immune regulation, and metabolic pathways. Some of these proteins may be associated with chronic inflammation and cardiovascular disease risk related to OSA. The study identified a range of candidate biomarkers, indicating that DIA mass spectrometry can sensitively detect protein expression changes and provide new directions for early diagnosis and precision medicine in OSA.

 



Wu, Y X. et al. Archivos de Bronconeumología, 2025.

Figure 2. DIA Quantitative Proteomic Analysis Process for Serum and Urine.

 

Deliverables

1. Experimental Procedure

 

2. Relevant Mass Spectrometry Parameters

 

3. Mass Spectrometry Images

 

4. Raw Data Files

 

5. Protein Differential Level Analysis

 

6. Bioinformatics Analysis

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!