DIA based Protein Quantitative Service

Data-independent acquisition (DIA) is a non-dependent data scanning mode, a holographic mass spectrometry data acquisition mode based on the electrostatic field orbitrap. After primary mass spectrometric detection, all ionized compounds of a given sample that fall within a specified mass range are fragmented in a systematic and unbiased fashion. The fragment ions of all precursor ions are collected, and quickly scaned within precursor isolation window to perform protein qualitative and quantitative analysis. Compared to data-dependent acquisition mode (DDA), DIA has better accuracy and reproducibility. The SWATH technique is a DIA technique that integrates the high-throughput detection of shotgun proteomics with the precise quantitative analysis of parallel reaction monitoring (PRM). It can quantify almost all detectable molecules in complex samples.

 

MtoZ Biolabs offers DIA quantitative proteomics service, including the AB SCIEX Triple-TOF 5600 plus high-resolution mass spectrometry system, which features fast scanning speed and quantitative sensitivity of tandem quadrupole mass spectrometry systems and integrates high resolution, accurate mass stability, high sensitivity, and high-speed scanning of the mass spectrometry system. You only need to send us your samples, and we will take care of all subsequent project matters, including protein extraction, protein digestion, peptide separation, mass spectrometric analysis, raw mass spectrometric data analysis, and bioinformatics analysis.

 

Applications

It can be applied to various bioscience research in agriculture, forestry, environment, food, and medicine to build biological sample database and discover biomarkers.

 

Service at MtoZ Biolabs

1. Experimental Procedures

2. Relevant Mass Spectrometric Parameters

3. Mass Spectrometric Images

4. Raw Data

5. Protein Differential Analysis

6. Bioinformatics Analysis