PCT-DIA Proteomics Service

Pressure cycling technology (PCT) is an efficient biological sample preparation technology and a patented technology developed by Pressure BioSciences Inc (PBI), USA. It uses pressure cycling between atmospheric pressure and ultra-high (liquid) pressure (35,000 PSI or higher), repeatedly breaking cells and enabling the extraction of important biomolecules such as proteins from various samples (cells and tissues from humans, animals, plants, and microorganisms). Data-independent acquisition (DIA) is a holographic mass spectrometry data acquisition mode based on the electrostatic field Orbitrap and is a data-independent scanning mode. Compared to data-dependent acquisition mode (DDA), DIA technology divides the entire scanning range of mass spectrometry into several windows, selecting, fragmenting, and detecting all ions within each window, providing better accuracy and repeatability. The combination of PCT and DIA technologies allows the study of the proteome of paraffin-embedded or formalin-fixed tissue samples and trace samples.

 

MtoZ Biolabs, using Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nanoliter chromatography technology, offers a PCT+PRM proteomics analysis service. You only need to send us your samples, and we will take care of all subsequent project procedures, including protein extraction, protease digestion, peptide separation, mass spectrometry analysis, raw data analysis, and bioinformatics analysis.

 

Analysis Workflow

 



Guo, T. et al. iScience. 2019.

Figure 1. PCT+DIA Proteomics

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Protein Differential Level Analysis

6. Bioinformatics Analysis