MtoZ Biolabs offers the CAR/TCR Gene Copy Number Detection Kit which is specifically designed for detecting the copy number of target genes, such as CAR/TCR genes, in human cell products prepared using common HIV-1 lentiviral vector technology. It uses multiplex PCR fluorescent probe method to efficiently and specifically detect gene copy numbers.
Product Overview
CAR/TCR genes typically exist in low copy numbers. Due to the scarcity of target gene copies and the uneven integration efficiency of lentiviral vectors, accurately detecting the copy number of these genes in various samples presents a significant challenge.
1. Difficulty in Extracting Low-Copy Genes
As CAR/TCR genes have a low copy number, if the nucleic acid recovery efficiency is not high during extraction, it may result in insufficient target gene quantity, affecting the accuracy and sensitivity of subsequent PCR amplification.
2. Sample Impurities Interfere with Amplification
Samples such as blood, cells, and tissues often contain host DNA, RNA, proteins, and other impurities, which may affect the PCR reaction, leading to weak or unstable detection signals.
3. High Demands for Multiplex PCR Amplification
In multiplex PCR detection, multiple target genes need to be amplified simultaneously. Variations in amplification efficiency among different genes can affect the accuracy and reliability of the results.
Advantages
✅ High Sensitivity and Specificity
Using the multiplex PCR-fluorescent probe method, it efficiently and specifically detects gene copy numbers, ensuring accurate identification even in low-copy samples.
✅ Optimized Nucleic Acid Extraction Conditions
Nucleic acid extraction conditions are optimized for cell and tissue samples, allowing efficient recovery of target genes and enhancing the sensitivity and accuracy of subsequent PCR amplification.
✅ Reduced Impurity Interference
Specially designed reagents and reaction systems minimize the interference from host DNA, RNA, and other impurities, improving the stability and consistency of detection results.
✅ Multiplex PCR Advantage
MtoZ Biolabs' CAR/TCR Gene Copy Number Detection Kit supports multiplex PCR amplification, enabling the simultaneous detection of multiple target genes, saving time and improving work efficiency.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
