The Coomassie Brilliant Blue R-250 from MtoZ Biolabs is a ready-to-use, high-sensitivity staining solution designed for SDS-PAGE gel visualization and downstream analytical workflows. The formulation enables clear detection of protein bands at the nanogram level within 20 minutes and is fully compatible with mass spectrometry-based protein identification and sequencing.
Product Overview
Coomassie staining is one of the most widely used visualization methods following protein electrophoresis. However, traditional staining workflows introduce several bottlenecks that reduce experimental efficiency and data quality:
1. Labor-Intensive and Time-Consuming Workflows
Traditional protocols require preparation and repeated exchange of fixative, staining, and destaining solutions. The full process typically takes several hours or even overnight, making rapid screening difficult.
2. Limited Sensitivity
Standard Coomassie methods exhibit poor sensitivity toward low-abundance proteins, with detection limits typically in the tens to hundreds of nanograms. Weak bands may not be detected, resulting in information loss.
3. Use of Toxic Reagents
Methanol and acetic acid are routinely used during staining and destaining, posing health hazards, producing strong odors, and increasing laboratory waste disposal burdens.
4. Poor Contrast and MS Interference
Conventional staining produces pale blue bands with low contrast that are difficult to image. Residual dye and fixatives may interfere with downstream mass spectrometry or protein sequencing by introducing unwanted crosslinking or chemical background.
Advantages
✅ Ready-to-Use and Rapid Staining
The Coomassie Brilliant Blue R-250 from MtoZ Biolabs is supplied as a ready-to-use working solution with no preparation required. Staining and rinsing can be completed in as little as 20 minutes, achieving clear visualization of single protein bands containing less than 6 ng of protein. This dramatically increases throughput for gel-based screening workflows.
✅ Ultra-High Sensitivity
The optimized formulation supports an optional enhancement workflow (~3 hours), enabling sensitivity down to <3 ng per band, positioning it among the most sensitive colorimetric staining reagents available for gel electrophoresis. Low-abundance proteins are reliably revealed without signal loss.
✅ Superior Imaging and Enhanced Laboratory Safety
Stained protein bands develop a vivid deep violet color with high contrast against the gel background, providing publication-grade images and improved performance on gel imaging systems and scanners. The entire workflow requires only aqueous rinses - no methanol or acidic fixatives - improving operator safety and reducing environmental impact.
✅ Full Compatibility with Downstream Mass Spectrometry
The product is specifically optimized for in-gel digestion and peptide extraction workflows, ensuring excellent compatibility with LC-MS/MS-based protein identification and sequencing. No residual chemicals introduce mass shifts, ion suppression, or modifications that compromise MS analysis.
✅ Reliable Performance and Long Shelf Life
The reagent maintains stability for up to 1 year at room temperature, enabling convenient storage without refrigeration. Performance consistency across batches supports reliable, reproducible experimental outcomes in proteomics and biochemical workflows.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
