HEK293T Host Cell Protein Standards

 

In proteomics research and biopharmaceutical quality control, standardized protein samples play a critical role in ensuring experimental accuracy, reproducibility, and comparability. Particularly in commonly used protein analysis techniques such as Western Blot, ELISA, and LC-MS, reference standards used as positive controls or quantitative benchmarks significantly enhance the reliability and comparability of experimental data.

 

The 293T cell line (HEK293T), a widely used human embryonic kidney cell line in biomedical research, is known for its high transfection efficiency and stable protein expression capability. Its well-defined origin and stable characteristics make 293T cell protein digests an ideal raw material for generating protein reference standards.

 

To meet the growing demand for high-quality standards in both research and industry, MtoZ Biolabs has developed HEK293T host cell protein standards based on its well-established protein sample preparation platform. These reference materials provide consistent and reliable controls for protein analysis experiments, helping improve experimental efficiency and reduce the cost of repeated trials.

 

Product Overview

Product Name: HEK293T host cell protein standards

Product Form: Lyophilized Powder / Solution

Source: Human Embryonic Kidney 293T Cells

Processing Method: Cell Lysis, Protein Extraction, Standardized Concentration Adjustment, Enzymatic Digestion, Desalting

Application: Reference Sample for Mass Spectrometry Analysis

 

This standard is prepared by the MtoZ Biolabs technical team using a unified processing workflow. It features controlled protein concentration and high consistency in peptide composition, making it suitable for various downstream proteomics applications. It demonstrates excellent reference value in instrument performance validation, method development, and quality control analysis.

 

Protocol

The usage of the HEK293T host cell protein standards is relatively simple and compatible with various routine experimental workflows. The following is the recommended protocol:

 

1. Sample Reconstitution

(1) It is recommended to reconstitute the lyophilized product using 0.1% formic acid aqueous solution or an appropriate MS-grade buffer.

(2) For the liquid formulation, use directly after opening. Avoid repeated freeze-thaw cycles.

 

2. Sample Loading

(1) LC-MS: The reconstituted peptide solution can be directly injected for analysis without additional enzymatic digestion.

 

3. Storage Recommendations

(1) Store at -80°C to extend shelf life.

(2) It is recommended to use it at one time after opening, or freeze it after packing to avoid repeated freeze-thawing.

 

Please adjust the concentration and volume appropriately based on the specific experimental setup to achieve optimal signal performance.

 

Features and Benefits

MtoZ Biolabs' HEK293T host cell protein standards offer multiple advantages in terms of quality control and experimental compatibility:

 

1. Unified Source with Defined Background  

HEK293T cells with stable passage history are used as the protein source, ensuring traceability and batch-to-batch consistency.

 

2. Standardized Digestion Workflow

Optimized trypsin digestion conditions ensure broad peptide coverage and high integrity, supporting various protein quantification and QC applications.

 

3. Strong Experimental Compatibility  

Serves as a reliable reference sample for MS-based identification and quantification, suitable for diverse proteomic workflows.

 

4. Easy to Operate and Ready-to-Use Design  

HEK293T host cell protein standards are provided in ready-to-use liquid or lyophilized form,  easy to handle and integrate into standard lab protocols.

 

5. Rigorous Quality Control for Consistency  

Each batch undergoes strict validation including SDS-PAGE, BCA protein quantification, and LC-MS testing to ensure reproducibility and consistent product quality.

 

Applications

The HEK293T host cell protein standards are broadly applicable to the following research and analytical fields:

 

1. Peptide Quantification Standard

Serves as a reference for calibration curve construction, enhancing the accuracy and reproducibility of peptide quantification results.

 

2. Mass Spectrometry Quantification Method Development

Supports method validation, platform performance evaluation, and peptide selection, particularly suitable for quantitative strategies such as DIA, PRM, and MRM.

 

3. Antibody Screening and Validation

Provides a consistent background of protein-derived peptides in antibody development or validation experiments, aiding in the assessment of antibody specificity.