Serine Hydrolase Probes

MtoZ Biolabs Serine Hydrolase Probes: Broad-Spectrum Capture of Active Serine Hydrolases and Investigation of Functional and Target Roles in Metabolic, Neurological, and Immune Regulation

Serine hydrolases constitute a large enzyme superfamily that includes proteases such as trypsin and thrombin, as well as esterases, lipases, and amidases. These enzymes participate in diverse biological processes, including metabolism, digestion, neuronal signaling, immune responses, and apoptosis. Designed around the conserved catalytic serine residue at the active site, the Serine Hydrolase Probes from MtoZ Biolabs enable covalent labeling of catalytically active enzymes and support global enrichment and functional annotation of active serine hydrolase populations. This approach supports identification of disease-associated hydrolases, high-throughput inhibitor screening, and evaluation of off-target effects during early drug development.

Advantages

Mechanism-Driven Broad-Spectrum Labeling

In the MtoZ Biolabs Serine Hydrolase Probes, covalent modification of the catalytic serine residue at the active site enables broad-spectrum labeling of diverse serine hydrolases. This capability supports systematic investigation of this large enzyme superfamily, which is difficult to analyze comprehensively using traditional methods.

Direct Detection of Active Sites

The chemical reactivity of the active site is directly interrogated, allowing dynamic monitoring of enzyme activity and identification of hydrolases activated under specific physiological or pathological conditions.

Efficient Inhibitor Screening and Characterization

Competition assays conducted through co-incubation with candidate inhibitors support evaluation of inhibitor selectivity and potency toward specific serine hydrolases or broader enzyme families, facilitating lead compound optimization and safety evaluation.

Multifunctional Downstream Analytical Paths

The enriched material is compatible with multiple downstream analytical readouts, including Western Blot for target-focused validation and mass spectrometry for unbiased identification to discover functional enzymes or reveal off-target effects, supporting both systems biology studies and early drug discovery research.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.