MtoZ Biolabs' Human Immunoglobulin M (IgM) ELISA Kit is a high-specificity immunoassay kit designed for quantitative detection of human IgM. It accurately measures IgM levels in serum, plasma, and other biological matrices. This kit is widely used in humoral immunity research, infectious disease diagnosis, autoimmune disorder investigation, and vaccine immunogenicity evaluation.
Product Overview
IgM is the first antibody produced during an immune response, making its accurate quantification essential for immune status assessment. Key detection challenges include:
1. Structural Complexity
IgM's pentameric structure (~900 kDa) can limit antibody accessibility to target epitopes, reducing capture efficiency.
2. Immunoglobulin Cross-Reactivity
High structural homology among IgG, IgA, IgE, and IgD increases the risk of isotype cross-reactivity and result overestimation.
3. Rheumatoid Factor Interference
RF present in patient sera can non-specifically bridge detection antibodies, generating false-positive signals.
4. Wide Concentration Range
IgM levels span from normal baseline to markedly elevated pathological states, requiring broad dynamic range coverage.
5. Matrix Effects
Hemolysis, lipemia, and high-protein matrices can compromise antigen-antibody interactions and signal accuracy.
Advantages
✅ Sensitivity
LOD reaches the ng/mL level, enabling reliable quantification of IgM at physiologically low concentrations and supporting early immune response monitoring.
✅ Specificity
Antibodies target epitopes unique to the IgM μ chain, with minimal cross-reactivity to IgG, IgA, IgE, and IgD, effectively excluding rheumatoid factor and matrix interference.
✅ Precision
Intra-assay (CV%<8%) and inter-assay (CV%<10%) CVs are within acceptable limits.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
