Whether you are characterizing the properties and functions of a specific protein, mapping protein-protein interaction networks, or precisely profiling post-translational modifications, an effective enrichment strategy is essential for generating high-quality data in downstream analyses. MtoZ Biolabs’ IP-MS Enrichment Product Line provides a complete suite of solutions, from targeted protein or complex purification to global modified-peptide capture, ensuring accurate isolation of your molecules of interest from complex biological samples. These enrichment tools establish a robust foundation for subsequent Western blotting or mass spectrometry-based identification and quantitative analysis.
Products
Target Protein/Complex Enrichment and Purification
MtoZ Biolabs' Target Protein/Complex Enrichment Solutions utilize high-specificity affinity materials, including Protein A/G, metal-chelation media, and biotin-streptavidin systems, to achieve efficient, low-background isolation of proteins of interest. These solutions are ideal for studying protein function, interaction partners, and multiprotein complex composition with high confidence.
Modified Protein/Peptide Enrichment
With dedicated technologies for phosphorylation, acetylation, lactylation, and other key post-translational modifications, MtoZ Biolabs’ PTM Enrichment Kits selectively capture modified peptides from complex digested samples. This high-performance enrichment significantly enhances PTM identification depth and site coverage in downstream mass spectrometry analyses.
Immunopeptidome Enrichment
Designed for the efficient isolation and purification of peptides presented by major histocompatibility complex (MHC) molecules, MtoZ Biolabs’ Immunopeptidome Enrichment Solutions offer high sensitivity and specificity. These tools provide advanced technological support for studies in tumor immunology, antigen discovery, and vaccine development.
Lab Equipment at MtoZ Biolabs
1. Refrigerated Centrifuge
The refrigerated centrifuge used in MtoZ Biolabs’ IP-MS enrichment workflow maintains low temperatures throughout the entire process, effectively preventing protein degradation and preserving the integrity of protein complexes and post-translational modifications. This ensures that enriched samples retain their biological activity and authenticity for downstream analyses.
2. Vortex Mixer
The vortex mixer incorporated into MtoZ Biolabs’ enrichment procedures provides efficient yet gentle mixing, greatly improving binding efficiency and reproducibility in immunoprecipitation and affinity-based enrichment workflows. Its ease of use helps save both time and labor while ensuring consistent results.
3. Microplate Reader
MtoZ Biolabs employs high-throughput microplate readers compatible with 96-well formats, enabling rapid, accurate detection with minimal sample volume. This instrument is essential for experimental standardization and quality control across the enrichment and quantification pipeline.
4. Vacuum Concentrator
The vacuum concentrator integrated into MtoZ Biolabs’ mass spectrometry sample-preparation workflow uses a gentle, low-temperature concentration process that prevents degradation or loss of modified peptides. With high processing throughput, it significantly enhances efficiency prior to LC-MS analysis.
Advantages
1. High Specificity and Enrichment Efficiency
MtoZ Biolabs’ IP-MS Enrichment Products feature carefully optimized resins and ligands that deliver high affinity and exceptional specificity toward target molecules, substantially improving signal-to-noise ratios and overall analytical confidence.
2. Outstanding Mass Spectrometry Compatibility
All reagents and workflows in MtoZ Biolabs’ Enrichment Kits are engineered to avoid MS-interfering contaminants. Mild elution conditions ensure that recovered proteins or peptides can be directly subjected to downstream LC-MS/MS analysis without additional cleanup steps.
3. High Reproducibility and Reliability
Pre-packed columns and ready-to-use kits from MtoZ Biolabs ensure standardized operation and excellent reproducibility, minimizing user-to-user and batch-to-batch variability.
4. Comprehensive Coverage of Research Needs
From single-protein enrichment to global post-translational modification profiling, and from classical signaling pathways to cutting-edge research areas, MtoZ Biolabs provides a complete, one-stop suite of enrichment solutions tailored to diverse proteomics applications.
Technical Support
Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
- Single Protein Enrichment KitsWhether investigating post-translational modifications or mapping the interaction network of a specific protein, efficient and specific enrichment of a target protein from complex samples is a critical first step. The MtoZ Biolabs Single Protein Enrichment Kits offer Protein A/G-based co-immunoprecipitation as well as multiple tagged protein purification options, supporting studies of endogenous proteins and enabling rapid purification of overexpressed tagged proteins, thereby ensuring high-purity protein……
- Modified Protein/Peptide Enrichment KitsProtein post-translational modifications (PTMs) regulate nearly all cellular life processes. The MtoZ Biolabs Modified Protein/Peptide Enrichment Kits product series encompasses both histone-modification research and comprehensive protein-modification profiling. Utilizing high-performance enrichment materials and methodologies, these kits specifically capture key modifications such as phosphorylation, acetylation, and lactylation, markedly increasing the coverage of modification-site identification and.....
- Immunoenrichment KitsMtoZ Biolabs Immunoenrichment Kits - Targeting the Core of Antigen Presentation, Enabling Efficient Capture of MHC Peptides and Providing a Critical Foundation for Tumor Immunology and Vaccine Development Immunopeptides presented by MHC molecules serve as the molecular link between intracellular protein states and adaptive immune responses. Comprehensive characterization of these peptides is essential for understanding immune surveillance, developing neoantigen-based vaccines, and advancing innovativ......