MtoZ Biolabs provides an integrated workflow covering SDS-PAGE separation and high-sensitivity staining, supporting reliable protein visualization, quantitative analysis, and downstream mass spectrometry-based identification. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used method for protein separation, molecular weight estimation, purity assessment, and sample preparation. Under reducing conditions, SDS denatures proteins and imparts uniform negative charges, enabling size-based migration and accurate separation within polyacrylamide gels. Following electrophoresis, protein bands are visualized using high-sensitivity staining techniques, after which individual bands can be excised and enzymatically digested for mass spectrometric identification. The electrophoresis and staining product line offered by MtoZ Biolabs encompasses pre-cast gels, electrophoresis buffers, staining reagents, and complete starter kits to ensure reproducible protein analysis and compatibility with downstream mass spectrometry workflows.
Products
Pre-cast Electrophoresis Gels
SDS-PAGE pre-cast gels are available in multiple acrylamide percentages, well formats, and gel dimensions, providing high resolution, batch-to-batch reproducibility, and operational convenience, while eliminating the variability associated with in-house gel preparation.
Electrophoresis Buffers
Ready-to-use buffer powders and concentrates maintain stable pH and ionic strength during electrophoresis, contributing to consistent protein migration and reliable analytical output.
Electrophoresis Staining Reagents
Coomassie Brilliant Blue rapid staining solutions, high-sensitivity silver staining kits, and fluorescent staining reagents (e.g., SYBR Ruby) are available to support workflows ranging from rapid screening to nanogram-level protein detection. Several staining formats are highly compatible with downstream mass spectrometry analysis.
Electrophoresis System Starter Kits
Each kit contains core consumables required for SDS-PAGE workflows (including pre-cast gels, buffer solutions, sample loading buffer, and pre-stained protein markers), making them suitable for new users and for laboratories implementing standardized electrophoresis procedures.
Lab Equipment at MtoZ Biolabs
Vertical Electrophoresis Chamber and Power Supply
Core equipment used to perform SDS-PAGE protein separation.
Gel Imaging System
Employed to document stained gel images and to support semi-quantitative analysis.
Gel Cutting Knife/Spot Picker
Utilized to precisely excise target protein bands or spots for downstream in-gel digestion workflows.
Advantages
Superior Separation Performance and Reproducibility
High-quality pre-cast polyacrylamide gels combined with optimized buffer systems yield sharp protein bands and high separation resolution with minimal batch-to-batch variation, supporting data comparability and analytical reliability.
Workflow-wide Staining Sensitivity and Compatibility
MtoZ Biolabs offers staining solutions ranging from economical and rapid Coomassie-based protocols to fluorescence staining approaches achieving sensitivity levels comparable to silver staining. Certain staining methods maintain high analytical sensitivity while minimizing adverse effects on downstream mass spectrometry-based protein identification.
Reduced Operational Complexity and Workflow Time
Ready-to-use pre-cast gels, buffer solutions, and staining reagents eliminate preparation and optimization steps, enabling researchers to allocate more effort toward experimental planning and data interpretation.
System Compatibility and Workflow Scalability
MtoZ Biolabs' products are compatible with commonly used electrophoresis systems and can be integrated with MtoZ Biolabs' in-gel digestion and peptide extraction product lines to establish an end-to-end workflow spanning protein separation, staining, and mass spectrometry sample preparation.
Technical Support
Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.