MtoZ Biolabs' Kinase Enrichment Kit with ATP Probe utilizes the powerful ActivX ATP probe to selectively label and enrich catalytically active kinases and ATPases, including chaperones and metabolic enzymes, for advanced activity-based profiling and inhibitor screening.
Product Overview
Studying the functional kinome and kinase inhibitors in their native cellular environment is challenging. The main challenges include:
1. Distinguishing Active Kinases from Abundant Proteins
Traditional methods only measure kinase abundance, not their active, catalytically competent states, making it difficult to assess functional kinase populations or their interactions with inhibitors.
2. Inefficient Inhibitor Screening and Quantification
Standard kinase assays often test only a single kinase at a time, leading to low throughput and limiting the ability to quantitatively assess inhibitor affinity and specificity across the kinome.
3. Missed Off-Target Kinase Interactions
Conventional assays are hypothesis-driven, often focusing on known kinases, which can miss potential off-target effects and other kinases involved in cellular processes, limiting the depth of inhibitor profiling.
4. Failure to Capture Inactive but Ligand-Bound Kinases
Many kinases remain bound to ATP or inhibitors in an inactive state, but traditional methods fail to capture this regulatory information, missing important insights into cellular signaling dynamics.
Advantages
✔️Active Site Directed, Broad Kinase Coverage
The ActivX ATP probe in MtoZ Biolabs' Kinase Enrichment Kit covalently labels conserved ATP binding sites, enabling selective capture of kinases and related ATP binding enzymes, including both active and inactive ATP bound forms, to provide a more complete and functionally relevant view of the kinome in native samples.
✔️Quantitative Inhibitor Screening
The kit's inhibitor competition feature allows researchers to quantitatively assess the binding affinity and target occupancy of small molecule inhibitors, streamlining drug development and screening efforts.
✔️Reversible and Gentle Enrichment Strategy
Desthiobiotin-based affinity capture allows efficient enrichment followed by mild elution, preserving protein and peptide integrity for downstream analysis.
✔️Seamless Integration with Orthogonal Readouts
Probe-labeled peptides and proteins can be enriched and are compatible with SDS-PAGE and Western blotting for targeted validation. They also support unbiased LC-MS/MS identification and quantification.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
