The iST Sample Preparation Kit from MtoZ Biolabs provides an integrated, standardized workflow for proteomics sample preparation, supporting efficient protein cleanup and digestion to generate LC-MS/MS ready peptides with strong reproducibility across samples.
Product Overview
Proteomics sample preparation can become the main source of variability in LC-MS/MS studies, especially when sample numbers grow, input is limited, or labeling and batching extend preparation timelines. Common constraints include:
1. Step-Driven Variability Across Batches
Multi-reagent, multi-tube workflows introduce timing and handling differences that can propagate into measurable run-to-run variation, complicating quantitative comparisons.
2. Contamination and MS Interference
Hydrophilic and hydrophobic contaminants, including salts, detergents, chaotropes, lipids, and nucleic acids, can elevate background, reduce ionization efficiency, and shorten column or instrument uptime.
3. Sample Loss During Transfers and Cleanup
Repeated handling, precipitation, and device changes increase adsorption and incomplete recovery, disproportionately affecting low-input or precious samples.
4. Scalability Limitations
As cohorts expand, maintaining consistent processing conditions becomes difficult without a standardized workflow that supports parallelization and automation.
Advantages
Designed for consistent, high-throughput proteomics workflows, the iST Sample Preparation Kit from MtoZ Biolabs provides the following benefits:
✅ Standardized, All-in-One Workflow
Consolidates core preparation steps into a single kit format to reduce reagent variability and simplify execution across operators and batches.
✅ Time-Efficient, Lab-Friendly Processing
Enables streamlined, hour-scale preparation with reduced hands-on effort, supporting faster turnaround without sacrificing data quality.
✅ Cleaner Peptides for LC-MS/MS Performance
Optimized cleanup reduces common MS-interfering components, helping improve signal quality and supporting stable LC-MS/MS operation.
✅ Reproducibility for Quantitative Proteomics
Controlled, repeatable conditions help minimize batch effects and improve comparability across time points, labels, and large cohorts.
✅ Manual to Automation-Ready Flexibility
Supports manual use as well as semi-automated or automated processing options, making it suitable for routine studies and higher-throughput pipelines.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
