The iST-NHS Sample Preparation Kit from MtoZ Biolabs streamlines MS sample preparation for amine-reactive (NHS-ester) chemical labeling of primary amines by providing labeling-compatible peptide processing and efficient cleanup to deliver consistent, LC-MS/MS ready peptides across low-input and large-cohort studies.
Product Overview
Chemical labeling strategies such as TMT and iTRAQ require controlled sample composition and reaction conditions to support high labeling efficiency and reliable quantitative performance. In practical proteomics pipelines, key constraints often include:
1. Labeling Performance Depends on Reaction Compatibility
Amine-reactive labeling is sensitive to pH, buffer composition, and competing nucleophiles, which can lead to under-labeling and reduced quantitative consistency if conditions are not well controlled.
2. Step-Heavy Workflows Increase Batch-to-Batch Variation
When sample preparation involves many separate steps and extended handling, small differences in timing and cleanup can accumulate into measurable batch effects, particularly in multiplexed studies.
3. Low-Input Samples Are More Vulnerable to Recovery Loss
Multiple transfers and separate cleanup steps can reduce peptide recovery, directly impacting identification depth and quantitative stability for limited or precious inputs.
4. Residual Contaminants May Reduce LC-MS/MS Robustness
Hydrophilic and hydrophobic interferents such as salts, detergents, and lipids may elevate background and suppress ionization, which can reduce sensitivity and increase maintenance burden in high-throughput runs.
Advantages
To support robust chemical labeling workflows at scale, the iST-NHS Sample Preparation Kit from MtoZ Biolabs delivers the following benefits:
✅ Built for Amine-Reactive Labeling Compatibility
Provides peptide handling conditions aligned with NHS-ester labeling workflows, supporting consistent labeling performance and improved quantitative comparability.
✅ Standardized, All-in-One Reagents
Consolidates key preparation needs into a single kit format to reduce reagent-to-reagent variability and simplify execution across operators and batches.
✅ Cleaner Peptides for Confident Quantification
Optimized cleanup reduces common MS interferents, helping improve signal quality and supporting stable LC-MS/MS operation in multiplexed studies.
✅ Reduced Handling to Protect Recovery
Streamlined processing minimizes transfer-driven loss, supporting improved peptide yield for low-input and high-throughput applications.
✅ Scalable Batch Processing
Designed to support parallel preparation across larger sample sets and can be adapted to manual or automation-enabled workflows depending on laboratory setup.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
