MtoZ Biolabs' Human Insulin (INS) ELISA Kit (Double Antibody Sandwich Method) is a high-sensitivity immunoassay kit based on the double antibody sandwich principle, specifically designed for the quantitative detection of human insulin. It enables precise capture and quantification of insulin levels across a variety of complex biological samples, including serum, plasma, and cell culture supernatants. This kit is widely used in diabetes mechanism research, pancreatic β-cell function assessment, and molecular studies of insulin resistance and metabolic syndrome.
Product Overview
Insulin plays a central role in blood glucose regulation; however, its accurate detection presents several technical challenges:
1. Extremely Low Concentration
Fasting serum insulin levels range only from 2–25 μIU/mL, placing exceptionally high demands on assay sensitivity.
2. Interference from Precursor Molecules
Proinsulin, C-peptide, and other structurally homologous molecules can cause cross-reactivity when antibody specificity is insufficient, leading to overestimation of true insulin levels.
3. Complex Sample Matrix
Heterophilic antibodies, hemolysis, lipemia, and insulin autoantibodies can all interfere with detection signals, resulting in false positives or false negatives.
4. Poor Stability
Insulin is susceptible to protease degradation, and repeated freeze-thaw cycles result in significant concentration loss, requiring strict sample handling protocols.
5. Wide Dynamic Range
Insulin concentrations span several orders of magnitude from physiological lows to pathological highs, making the assay prone to the Hook Effect.
Advantages
✅ High Sensitivity
Utilizing affinity-optimized antibody pairs combined with an HRP-based signal amplification system, the assay achieves a limit of detection (LOD) at the sub-pmol/L level, ensuring reliable quantification even at minimal insulin concentrations.
✅ Wide Dynamic Range
The standard curve covers a broad concentration range from physiological lows to pathological highs, effectively eliminating the Hook Effect and reducing the need for additional sample dilution in most applications.
✅ Low Cross-Reactivity
The kit specifically excludes interference from insulin precursor molecules and insulin analogs, delivering highly reliable and accurate results with confidence.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
