DTSSP (3,3′-dithiobis(sulfosuccinimidyl propionate)) from MtoZ Biolabs is a water-soluble, thiol-cleavable, amine-reactive homobifunctional crosslinker designed for reversible stabilization of protein interactions in aqueous and cell-surface-focused workflows.
Product Overview
In protein interaction studies where aqueous conditions and surface specificity are required, crosslinker solubility, membrane permeability, and controlled cleavability are critical design considerations. Among amine-reactive crosslinkers, several inherent factors often influence experimental outcomes:
1. Limited Aqueous Compatibility
Crosslinkers that require organic solvents for dissolution may disrupt protein structure or complicate workflows intended to remain fully aqueous.
2. Lack of Controlled Reversibility
Non-cleavable linkers permanently fix protein complexes, limiting flexibility in workflows that require subsequent dissociation or detailed component analysis.
3. Unintended Intracellular Reactivity
Membrane-permeable crosslinkers may modify intracellular proteins when selective cell-surface or extracellular crosslinking is desired.
4. Constraints in Downstream Processing
Crosslinkers without defined reduction-sensitive bonds may limit compatibility with reducing conditions commonly used in electrophoretic and proteomics-based analyses.
Advantages
The MtoZ Biolabs DTSSP (3,3′-dithiobis(sulfosuccinimidyl propionate)) offers the following advantages:
✅ Water-Soluble Sulfo-NHS Ester Chemistry
DTSSP is readily soluble in aqueous buffers and reacts efficiently with primary amines, enabling direct use without organic solvents.
✅ Thiol-Cleavable Disulfide Spacer
The central disulfide bond can be selectively cleaved under reducing conditions, allowing controlled reversal of crosslinks during downstream analysis.
✅ Amine-Specific Reactivity
Homobifunctional sulfo-NHS ester groups react selectively with primary amines, including lysine side chains and protein N-termini, forming stable amide bonds under mild conditions.
✅ Surface-Oriented Crosslinking Control
The charged sulfonate groups limit membrane permeability, making DTSSP well-suited for cell-surface and extracellular protein crosslinking applications.
✅ Compatibility With Standard Analytical Workflows
DTSSP-crosslinked samples can be efficiently processed under reducing conditions for SDS-PAGE, immunoblotting, immunoprecipitation, and mass spectrometry-based analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
