MtoZ Biolabs 0.1% Trifluoroacetic Acid (v/v), LC-MS Grade is a ready-to-use ion-pairing mobile-phase additive designed for chromatography-first RP-LC separations of peptides and proteins, improving peak shape and retention stability in routine analyses and method development. This formulation is ideal for peptide mapping, RP-HPLC separations, fraction collection prior to LC-MS, and method optimization.
Product Overview
In peptide and protein separations, small variations in mobile-phase composition can significantly impact chromatographic performance, especially when consistency across multiple injections is critical:
1. Peak Tailing and Adsorption
Peptides, particularly those with basic residues, can interact with residual silanol groups or active sites in LC systems, causing peak broadening and reduced resolution.
2. Co-Elution in Complex Samples
Wide hydrophobicity differences and high sample complexity may cause peak crowding and co-elution, limiting identification depth and quantitation accuracy.
3. Retention Time Drift and Method Instability
Manual preparation can introduce concentration errors or contamination, leading to retention time variability and compromising cross-batch consistency.
4. Background Interference
Impurities in the mobile phase can generate ghost peaks and elevate baseline noise, reducing confidence in low-level signals.
5. MS Sensitivity Trade-Offs
While TFA enhances chromatographic performance, uncontrolled usage may suppress ESI response. Proper concentration and MS-friendly impurity control are essential.
Advantages
✅ Validated
Compatible with mainstream RP-LC conditions and LC-MS workflows, supporting routine proteomics runs, method development, and improving cross-batch consistency.
✅ Purified
Tightly controlled impurities, including metal ions (<50 ppb), minimize ghost peaks and unwanted adduct-related interference.
✅ Convenient
A ready-to-use 0.1% (v/v) formulation that reduces weighing errors and contamination risks associated with in-house preparation.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
