Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) from MtoZ Biolabs is a water-soluble, heterobifunctional crosslinker with NHS ester and maleimide groups, designed for efficient amine-to-sulfhydryl conjugation, supporting protein labeling, crosslinking, and bioconjugate formation in biochemical and proteomics research.
Product Overview
In protein conjugation workflows, selecting the right crosslinker ensures efficient and specific conjugation while maintaining compatibility with downstream applications. Several challenges are commonly encountered in amine-to-sulfhydryl conjugation:
1. Limited Selectivity in Conjugation
Homobifunctional crosslinkers may result in random conjugation or self-crosslinking, reducing control over product homogeneity and orientation.
2. Need for Reversibility
Non-cleavable crosslinkers permanently fix proteins together, making it difficult to study protein complex disassembly or dissociation during subsequent analysis.
3. Compatibility with Aqueous Systems
Some crosslinkers require organic solvents for dissolution, complicating protein conjugation in biological or physiological environments.
4. Intracellular Crosslinking
Membrane-permeable crosslinkers are essential for modifying proteins within living cells or across membranes without affecting cell viability.
Advantages
The MtoZ Biolabs Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) provides the following advantages:
✅ Amine-to-Sulfhydryl Specificity
The NHS ester group efficiently reacts with primary amines, while the maleimide group selectively targets sulfhydryl groups, enabling precise and directional conjugation.
✅ Water-Soluble for Physiological Conditions
Sulfo-SMCC dissolves easily in water, making it ideal for conjugation reactions in aqueous or cell-based systems without the need for organic solvents.
✅ Stable Conjugation with Reversible Cleavage
The disulfide bond within the spacer arm can be cleaved under reducing conditions, allowing reversible conjugation and controlled product separation for downstream analysis.
✅ Membrane-Permeable for Intracellular Studies
The sulfonate group enhances membrane permeability, enabling intracellular protein modification and labeling applications.
✅ Compatible with Standard Analytical Techniques
Sulfo-SMCC-crosslinked conjugates can be readily analyzed by SDS-PAGE, immunoblotting, and mass spectrometry, supporting a wide range of downstream assays.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
