Subcellular Protein Extraction Kits

MtoZ Biolabs Subcellular Protein Extraction Kit - Optimized for Efficient and Selective Isolation of Subcellular Proteins from Various Cellular Compartments

Subcellular compartmentalization is fundamental to cellular organization and function. Proteins within different cellular compartments, such as the nucleus, cytoplasm, mitochondria, and membranes, exhibit distinct roles and interactions that are crucial to cellular processes. Analyzing these proteins requires specialized extraction strategies that preserve the integrity of their native conformations and associated functions. MtoZ Biolabs' Subcellular Protein Extraction Kit is meticulously designed to extract high-quality proteins from various subcellular compartments, ensuring reliable performance for targeted proteomic and functional analyses, including Western blotting (WB), co-immunoprecipitation (Co-IP), mass spectrometry (MS), and enzyme activity assays.

Advantages

Targeted Extraction of Subcellular Compartments

The MtoZ Biolabs Subcellular Protein Extraction Kit utilizes optimized lysis buffers that enable efficient and selective isolation of proteins from specific cellular compartments, including the nucleus, mitochondria, cytoplasm, and membrane fractions. This customization ensures minimal cross-contamination between compartments, delivering highly purified subcellular protein samples for precise analysis.

Preserved Protein Integrity and Functionality

With a focus on preserving protein integrity, the kit employs a gentle yet effective detergent-based system that minimizes protein degradation and aggregation. This approach is particularly beneficial for studies involving protein activity, protein–protein interactions, and post-translational modifications, ensuring that functional assays remain accurate and reproducible.

High-Yield Protein Recovery

Designed to maximize protein yield, this kit facilitates complete extraction while maintaining the protein profiles of each subcellular compartment. This high recovery rate is critical for downstream applications such as quantitative proteomics, enzyme activity assays, and multi-omics studies.

Comprehensive Compatibility with Analytical Techniques

The extraction kit is fully compatible with a wide range of downstream applications, including protein quantification (Bradford/BCA assays), SDS-PAGE, Western blotting, mass spectrometry, and enzyme activity assays. The streamlined process reduces the need for buffer exchange steps and minimizes potential sample loss.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.