MtoZ Biolabs' Golgi Apparatus Active Protein Extraction Kit is specially designed to extract active proteins from high-fat, high-viscosity Golgi apparatus samples, effectively overcoming the extraction challenges of traditional methods. The kit is widely used in membrane protein and signal transduction research.
Product Overview
The Golgi apparatus, as an important organelle, is rich in lipids and membrane proteins and has a tightly packed structure with abundant glycosylation, presenting unique challenges in protein extraction:
1. High Fat Content
The high lipid content in the Golgi apparatus makes it difficult for conventional extraction methods to separate lipids from proteins, leading to low extraction efficiency.
2. High Viscosity
Golgi samples are often highly viscous, making the lysis process difficult and affecting the integrity of the extracted proteins.
3. Complex Glycan Background
The glycosylation present in Golgi samples interferes with protein extraction, leading to protein degradation or incomplete extraction.
Advantages
✅ Optimized Lysis System
Combining mechanical disruption with specialized lysis reagents to efficiently break down the cellular membranes and release Golgi proteins.
✅ Low Viscosity and Clear Protein Solution
By applying viscosity-reducing agents and glycan removal reagents, the kit significantly reduces sample viscosity and ensures a smooth protein extraction process.
✅ Protein Stability Protection
Built-in protease inhibitors effectively prevent protein degradation, ensuring the activity and integrity of the extracted proteins.
✅ High Adaptability
The proteins extracted using MtoZ Biolabs' Golgi Apparatus Active Protein Extraction Kit can be used in downstream protein research experiments such as Western blotting, protein electrophoresis, immunoprecipitation, ELISA, and enzyme activity assays.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
