The C18 HPLC Column from MtoZ Biolabs is a high-capacity, high-resolution reversed-phase chromatography column that delivers superior separation performance and sensitive detection of low-abundance peptides in complex proteomic samples.
Product Overview
During LC-MS/MS-based proteomics, biomarker discovery, and systems biology studies, low-abundance peptides in complex biological samples are often difficult to capture and identify efficiently, and traditional chromatography columns typically face the following challenges:
1. Limited Loading Capacity and Masking of Low-Abundance Peptides
Conventional columns have relatively low loading capacity; when analyzing complex peptide mixtures, highly abundant peptides can saturate the stationary phase and prevent low-abundance peptides from being effectively retained and separated, ultimately reducing detection sensitivity.
2. Insufficient Separation Efficiency and Resolution
Poor column efficiency or selectivity may cause peptide co-elution, lowering MS identification depth and quantitative accuracy, and particularly compromising the analysis of post-translationally modified peptides.
3. Limited System Compatibility and Inconvenient Operation
Traditional column connection formats often require tools and introduce dead volume, making installation cumbersome while offering limited pressure tolerance, which can negatively impact method reproducibility and system stability.
4. Solvent Compatibility Limitations Affecting MS Sensitivity
Certain stationary phases or column chemistries rely heavily on ion-pairing reagents such as trifluoroacetic acid (TFA), which can suppress ionization in mass spectrometry and significantly reduce detection sensitivity.
Advantages
✅ High-Capacity Column Bed Optimized for High-Sensitivity Proteomics
The C18 HPLC Column from MtoZ Biolabs employs a uniquely engineered stationary phase and high-purity silica substrate to substantially increase column loading capacity. This enables efficient capture and separation of low-abundance peptides in highly complex samples, providing exceptional detection sensitivity in LC-MS workflows and supporting deep proteome coverage for both identification and quantitative analysis.
✅ Excellent Separation Efficiency and Superior Resolution
The column delivers optimized chromatographic performance, achieving sharp peak shapes and stable retention times without requiring TFA or under low-TFA conditions. The enhanced separation efficiency increases peptide identification depth and improves resolution of post-translationally modified peptides, making it suitable for biomarker discovery and systems biology research.
✅ Tool-Free, Zero-Dead-Volume nanoViper™ Connections
The column is pre-fitted with nanoViper™ hand-tightened fittings, enabling nearly zero-dead-volume connections without the need for tools. Installation is simple and robust, with pressure tolerance up to 1000 bar and broad compatibility with most nano-LC systems and third-party valve modules, ensuring high method reproducibility and system stability.
✅ Flexible Formats for Diverse Experimental Workflows
Multiple column lengths (50, 150, 250, and 500 mm) and internal diameters (50, 75, 300, and 1000 µm) are available to accommodate different sample loads, throughput requirements, and separation goals, supporting both high-throughput screening and high-resolution deep proteomic analyses.
✅ Broad Compatibility Across Peptide Analysis Applications
The column supports high-resolution chromatographic analysis of tryptic peptides, endogenous peptides, and synthetic peptides. It is suitable for protein identification, post-translational modification studies, quantitative proteomics, and metabolomics workflows across a wide range of LC-MS/MS platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
