The In-Gel Staining Kit launched by MtoZ Biolabs is specifically designed for protein visualization within gels, enabling stable and uniform staining while reducing background interference, and supporting electrophoresis result interpretation and downstream mass spectrometry studies.
Product Overview
In-gel staining is mainly applied to protein samples after electrophoretic separation. The limited sample amount, concentrated signals, and the need to preserve structural information pose specific challenges to staining sensitivity and compatibility.
1. Limited Staining Uniformity
Proteins are immobilized within a solid-phase matrix, which restricts dye penetration and binding efficiency, often resulting in insufficient or uneven signal development.
2. Compressed Signal Dynamic Range
When protein abundance spans a wide range, strong and weak signals are difficult to visualize simultaneously, affecting comprehensive data interpretation.
3. High Difficulty in Background Control
Non-specific adsorption or incomplete destaining can lead to residual background, compromising band boundary clarity and imaging stability.
4. Limited Compatibility with Downstream Operations
If the staining system lacks sufficient compatibility, it may interfere with subsequent digestion, identification, or quantitative analyses.
Advantages
✅ Optimized for Solid-Phase Samples
The staining system is better adapted to the distribution characteristics of proteins in a solid-phase environment, improving staining efficiency and consistency.
✅ Enhanced Visibility of Weak Signals
Helps improve the visualization of low-abundance proteins while avoiding overdevelopment of high-abundance bands, resulting in a more balanced overall protein distribution.
✅ Stable Background Control
The matched staining and washing conditions effectively reduce non-specific dye retention, producing clear, stable, and reproducible imaging results.
✅ Compatible with Downstream Analyses
The in-gel staining process of the MtoZ Biolabs in-gel staining kit does not introduce interfering modifications, allowing direct downstream workflows such as digestion and mass spectrometry analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
