Protein Demodification Kits (Pre-Digestion)

MtoZ Biolabs Protein Demodification Kits (Pre-Digestion): Efficient Removal of N-Linked Glycans for Reliable Pre-Digestion Sample Preparation in Glycoprotein Analysis and Structural Studies

In glycoproteomics and structure-function research, removal of glycosylation modifications prior to enzymatic digestion represents a critical pretreatment step for accurate mass spectrometry analysis and structure-activity relationship studies. The Protein Demodification Kits (Pre-Digestion) from MtoZ Biolabs provide high-purity recombinant PNGase F specifically designed for pre-digestion removal of N-linked glycans. The kits support processing of glycoprotein samples under either native or denaturing conditions, enabling complete glycan removal and yielding clean, reliable protein samples for downstream mass spectrometry, chromatographic, and related analytical workflows.

Advantages

High-Purity Enzyme Preparation

Within the MtoZ Biolabs Protein Demodification Kits (Pre-Digestion), high-purity recombinant PNGase F is supplied without contamination from endoglycosidase F1, F2, or F3, effectively preventing non-specific cleavage and ensuring analytical accuracy.

Complete Glycan Removal

All major types of N-linked glycans are efficiently hydrolyzed, enabling complete release of core oligosaccharides while preserving glycan structural integrity, which supports subsequent glycan profiling.

Broad Applicability

The kits are compatible with glycoproteins processed under native as well as denaturing conditions, accommodating diverse sample types and experimental requirements.

Strong Downstream Compatibility

The enzymatic reaction proceeds under mild conditions without affecting protein sequence or structural integrity, allowing seamless integration with downstream analyses including mass spectrometry identification, HPLC, and lectin microarray assays.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.